Amodiaquine dihydrochloride dihydrate
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Amodiaquine dihydrochloride dihydrate is a potent inhibitor of histamine N-methyltransferase (HNMT), with estimated Ki of 18.6 nM .
HNMT occurs ubiquitously in vertebrate species, and is widely expressed in mammalian tissues with particularly high expression levels in kidney, liver, colon, prostate, ovary, and spinal cord cells. HNMT is a cytosolic protein that is responsible for the intracellular inactivation of histamine .
Over the concentration range 10-3 ~ 10-8 M, amodiaquine was a significantly more potent inhibitor than other compounds including chlorhexidine, producing 80% inhibition on rat kidney HNMT at 10-6 M whilst the next best inhibitor at this concentration, chlorhexidine, produced only 27% inhibition. .
In mice with Propionibacterium acnes-primed and lipopolysaccharide-induced hepatitis, the subcutaneous injection of amodiaquine at 2 and 5 mg/kg significantly increased the histamine levels in the liver. Pretreatment with amodiaquine also improved the survival rate of the hepatitis mice, which was partially associated with the decrease in serum levels of aspartate aminotransferase and alanine aminotransferase .
. Horton J R, Sawada K, Nishibori M, et al. Structural basis for inhibition of histamine N-methyltransferase by diverse drugs. Journal of Molecular Biology, 2005, 353(2): 334-344.
. Harle D G, Baldo B A. Structural features of potent inhibitors of rat kidney histamine N-methyltransferase. Biochemical Pharmacology, 1988, 37(3): 385-388.
. Yokoyama A, Mori S, Takahashi H K, et al. Effect of amodiaquine, a histamine N-methyltransferase inhibitor, on, Propionibacterium acnes and lipopolysaccharide-induced hepatitis in mice. European Journal of Pharmacology, 2007, 558(1-3): 179-184.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||≥70.4mg/mL in DMSO|
|Chemical Name||4-((7-chloroquinolin-4-yl)amino)-2-((diethylamino)methyl)phenol dihydrochloride dihydrate|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|