Alisol B 23-acetate
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Alisol B 23-acetate is an agonist of farnesoid X receptor (FXR) .
FXR is a member of the nuclear receptor superfamily of ligand-activated transcription factors, playing a critical role in liver regeneration. Alisol B 23-acetate activates FXR-mediated regulation of genes involved in hepatocyte proliferation, potentially becoming a novel therapeutic option for facilitating efficient liver regeneration in patients subjected to liver resection .
In HepG2 cells transiently cotransfected with FXR expression plasmid, and FXR target gene, bile salt export pump (BSEP) promoter reporter vector, Alisol B 23-acetate at doses of 0.1, 1 and 10 μM significantly increased luciferase activity. Additional increases were detected in cells treated with higher doses and reached a highest increase (4.3-fold) in cells treated with 10 μM of Alisol B 23-acetate .
In male C57BL6 mice received partial hepatectomy, Alisol B 23-acetate treatment (12.5, 25 or 50 mg/kg/d, p.o., for 7 days) significantly increased the ratio of liver regrowth, in a dose-dependent manner. In addition, significant increases in hepatocyte DNA synthesis and mitotic index could also be observed in Alisol B 23-acetate-treated mice, indicating that Alisol B 23-acetate promoted liver regeneration via enhancing hepatocyte proliferation .
. Meng Q, Chen X, Wang C, et al. Alisol B 23-acetate promotes liver regeneration in mice after partial hepatectomy via activating farnesoid X receptor. Biochemical Pharmacology, 2014, 92(2): 289-298.
|Storage||Store at -20°C|
|Synonyms||Alisol B 23-monoacetate; Alisol B monoacetate|
|Solubility||≥51.5 mg/mL in DMSO; insoluble in H2O; ≥54.1 mg/mL in EtOH|
|Chemical Name||[1-(3,3-dimethyloxiran-2-yl)-3-[(8S,10S,11S,14R)-11-hydroxy-4,4,8,10,14-pentamethyl-3-oxo-1,2,5,6,7,9,11,12,15,16-decahydrocyclopenta[a]phenanthren-17-yl]butyl] acetate|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|
Quality Control & MSDS
- View current batch:
Purity = 98.66%
- COA (Certificate Of Analysis)
- MS (Mass Spectrometry)
- NMR (Nuclear Magnetic Resonance)
- MSDS (Material Safety Data Sheet)