In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
AH 6809 is an antagonist of prostaglandin receptor EP1, EP2 and D2 .
In COS-7 cells transfected with human EP2 receptor, AH 6809 treatment selectively inhibited the cAMP production increased by PGE2 but not forskolin. In the non-small cell lung cancer NCI-H1299 cell line, AH 6809 inhibited the binding of 3H-PGE2 to the cell membrane with IC50 value of 5 μM. The compound also inhibited the increase in cAMP level caused by PGE2 in NCI-H1299 cells. Moreover, AH 6809 significantly suppressed the cell proliferation and colony formation at low micromolar concentration. Besides that, AH 6809 exerted to be a specific DP-receptor blocking drug. It inhibited the anti-aggregation activity of PGD2 in whole blood [1, 2 and 3].
1. Woodward D F, Pepperl D J, Burkey T H, et al. 6-isopropoxy-9-oxoxanthene-2-carboxylic acid (AH 6809), a human EP2receptor antagonist. Biochemical pharmacology, 1995, 50(10): 1731-1733.
2. Qian X, Zhang J, Liu J. Tumor-secreted PGE2 inhibits CCL5 production in activated macrophages through cAMP/PKA signaling pathway. Journal of Biological Chemistry, 2011, 286(3): 2111-2120.
3. Ito S, Okuda E, Sugama K, et al. Evaluation of ZK110841 and AH6809, an agonist and an antagonist of prostaglandin DP-receptors on human platelets, with a PGD2-responsive cell line from bovine embryonic trachea. British journal of pharmacology, 1990, 99(1): 13-14.
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Solubility||Soluble in DMSO|
|Chemical Name||9-oxo-6-propan-2-yloxyxanthene-2-carboxylic acid|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|