In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Adenosine dialdehyde (AdOx) has been identified as an indirect methyltransferase inhibitor.
Histone methyltransferases are a group of enzymes that catalyze the methylation of histone lysine and arginine by adding methyl groups to specific histone arginine or lysine residues.
In vitro: Previous cell-based study showed that the treatment of cells with the methyltransferase inhibitor adenosine dialdehyde (AdOx) could lead to cell cycle arrest and death in different tested cell types. In addition, the e form of cell death and phenotypical outcom was found to be strikingly dependent on the concentration of AdOx. Results showed that lower AdOx concentrations could result in a G2 arrest and predominantly cause apoptosis, which was measured by biochemical and morphological criteria. In contrast, higher concentrations of AdOx led to a novel and so far undescribed form of cell death that was characterized by distinct, caspase-independent alterations of the cell shape with a marked protuberation of the nucleus, actin aggregation, cytoplasmic extensions, as well as incomplete chromatin condensation .
In vivo: So far, there is no animal data reported.
Clinical trial: Up to now, AdOx is still in the preclinical development stage.
 Schwerk C, Schulze-Osthoff K. Methyltransferase inhibition induces p53-dependent apoptosis and a novel form of cell death. Oncogene.2005 Oct 27;24(47):7002-11.
|Physical Appearance||A powder|
|Storage||Store at -20°C|
|Solubility||Soluble in DMSO|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|