In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Actinonin is an antibiotic and CD13/aminopeptidase N (APN) inhibitor. Actinonin is a naturally occuring antibacterial agent produced by antinomycete with potent deformylase inhibitory effect .
In vitro: Actinonin showed an inhibitory effect in cell growth. The in vitro IC50 values of actinonin against NB4, HL6O human cell lines, AKR mouse leukemia cells CD13-negative cell lines RA.JI and DAUDI human lymphoma were about 2-5 μg/ml. Cell cycle analysis indicated that actinonin induced a G1 arrest in NB4 and HL6O cells. Intracellular flow cytometry showed that actinonin could induce cell apoptosis in 20-35% of the cells . Actinonin dose-dependently inhibited the three forms (Zn-, Ni-, and Fe-) of peptide deformylases from both S. aureus and E. coli bacteria. The IC50 values of actinonin were 90, 3, 0.8, and 11 nM for Zn-PDF (E. coli), Ni-PDF (E. coli), Fe-PDF (E. coli), and Ni-PDF (S. aureus), respectively . Actinonin is a tight binding inhibitor of E. coli Ni-PDF with a Ki of 0.3 nM .
In vivo: Actinonin showed dose-dependent antitumor effects on AKR leukemia, resulting in a survival advantage. In the syngeneic AKR mouse leukemia model, treatment with 100 p.g actinonin daily for 3 days beginning at day 3 after transplantation showed significant antitumor effects .
. Gordon J J, Kelly B K, Miller G A. Actinonin: an antibiotic substance produced by an actinomycete[J]. Nature, 1962, 195: 701-702.
. Xu Y, Lai L T, Gabrilove J L, et al. Antitumor activity of actinonin in vitro and in vivo[J]. Clinical cancer research, 1998, 4(1): 171-176.
. Chen D Z, Patel D V, Hackbarth C J, et al. Actinonin, a naturally occurring antibacterial agent, is a potent deformylase inhibitor[J]. Biochemistry, 2000, 39(6): 1256-1262.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||≤30mg/ml in ethanol;30mg/ml in DMSO;30mg/ml in dimethyl formamide|
|Canonical SMILES||O=C([[email protected]](C(C)C)NC([[email protected]](CCCCC)CC(NO)=O)=O)N1CCC[[email protected]]1CO|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|