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Acetylcysteine Antioxidant;mucolytic agent

Catalog No.A8356
Size Price Stock Qty
10mM (in 1mL DMSO)
$60.00
In stock
5g
$50.00
In stock
10g
$60.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

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Sample solution is provided at 25 µL, 10mM.

Quality Control

Chemical structure

Acetylcysteine

Protocol

Cell experiment [1]:

Cell lines

PC12 cells

Preparation method

The solubility of this compound in DMSO is > 10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

1–1000 μM; 3h

Applications

In PC12 cells, Acetylcysteine (NAC) produced concentration-dependent decreases in DOPAL levels. Co-incubation of NAC (1-10 μM) with selegiline (1 μM) attenuated or prevented the Cys-DA response to selegiline, without interfering with the selegiline-induced decrease in DOPAL production or inhibiting tyrosine hydroxylation.

Animal experiment [2]:

Animal models

the R6/1 transgenic mouse model of Huntington’s disease (HD)

Dosage form

500 mg/kg i.p., 4 weeks

Application

In the R6/1 transgenic mouse model of Huntington’s disease (HD), Acetylcysteine (NAC)-HD mice showed forced-swim test (FST) immobility times intermediate to saline-HD and saline-WT mice. NAC exhibited antidepressant-like effect. HD mice co-administered with CPG (inhibitor of GLT-1) and NAC showed higher immobility times than NAC-HD mice, suggesting that the antidepressant-like effect of NAC is dependent on glutamate transport.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Goldstein DS1, Jinsmaa Y2, Sullivan P2, et al. N-Acetylcysteine Prevents the Increase in Spontaneous Oxidation of Dopamine During Monoamine Oxidase Inhibition in PC12 Cells. Neurochem Res. 2017 Aug 24.

[2] Wright DJ1,2, Gray LJ3,2, Finkelstein DI1, et al. N-acetylcysteine modulates glutamatergic dysfunction and depressive behavior in Huntington's disease. Hum Mol Genet. 2016 Jul 15;25(14):2923-2933.

Acetylcysteine Dilution Calculator

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Chemical Properties

Cas No. 616-91-1 SDF Download SDF
Synonyms N-acetylcysteine; N-acetyl-L-cysteine; NAC; Acetadote
Chemical Name (2R)-2-acetamido-3-sulfanylpropanoic acid
Canonical SMILES CC(=O)NC(CS)C(=O)O
Formula C5H9NO3S M.Wt 163.19
Solubility ≥8.2mg/mL in DMSO Storage Store at -20°C
Shipping Condition Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Background

Acetylcysteine is a derivative of cysteine; an acetyl group that is attached to the nitrogen atom. This compound is sold as a dietary supplement commonly claiming antioxidant and liver protecting effects.