In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Ac-YVAD-CHO is a potent, specific, reversible inhibitor of caspase-1, completely blocking caspase-1 activity (99%) at the concentration of 1 μM .
Caspase-1 is an evolutionarily conserved enzyme that proteolytically cleaves other proteins, the activation of which results in the processing and release of cytokines, interleukin 1β (IL-1β) and interleukin 18 (IL-18), as well as pyroptosis, an immunogenic form of cell death .
In THP-1 cells treated with α-hemolysin, Ac-YVAD-CHO inhibited 99% of the activity induced by hemolysin at 1 μM . In xanthine/xanthine-oxidase (X/XO)-treated G85R-expressing N2a cells, Ac-YVAD-CHO at 100 μM, protected G85R-expressing cells against X/XO-induced loss of cell viability, caspase-1 cleavage, and increased IL-1β secretion .
In toluene diisocyanate (TDI)-exposed mice, Ac-YVAD-CHO (1 mg/kg, i.n., q.d., for 7 consecutive days) effectively inhibited airway hyperresponsiveness (AHR), airway inflammation around the airways and epithelial goblet cell hyperplasia. In addition, Ac-YVAD-CHO robustly decreased the numbers of TH1/TH2 cells, and lowered levels of IL-18 and IL-1β .
. O'Brien M, Moehring D, Muñoz-Planillo R, et al. A bioluminescent caspase-1 activity assay rapidly monitors inflammasome activation in cells. Journal of Immunological Methods, 2017, 447: 1-13.
. Pasinelli P, Borchelt D R, Houseweart M K, et al. Caspase-1 is activated in neural cells and tissue with amyotrophic lateral sclerosis-associated mutations in copper-zinc superoxide dismutase. Proceedings of the National Academy of Sciences of the United States of America. 1998, 95(26): 1576-15768.
. Chen S, Yao L, Huang P, et al. Blockade of the NLRP3/caspase-1 axis ameliorates airway neutrophilic inflammation in a toluene diisocyanate induced murine asthma model. Toxicological Sciences, 2019, 170(2): 462-472.
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Solubility||Soluble in DMSO|
|Chemical Name||(4S,7S,10S,13S)-13-formyl-4-(4-hydroxybenzyl)-7-isopropyl-10-methyl-2,5,8,11-tetraoxo-3,6,9,12-tetraazapentadecan-15-oic acid|
|Canonical SMILES||CC(N([[email protected]](C(N([[email protected]](C(N([[email protected]](C(N([[email protected]](C([H])=O)CC(O)=O)[H])=O)C)[H])=O)C(C)C)[H])=O)CC1=CC=C(C=C1)O)[H])=O|
|Shipping Condition||Ship with blue ice, or upon other requests.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.|