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Ac-YVAD-CHO

Catalog No.
A8954
Selective inhibitor of interleukin-1β converting enzyme (ICE; Caspase-1)
Grouped product items
SizePriceStock Qty
1mg
$62.00
Ship with 5-10 days
5mg
$250.00
Ship with 5-10 days
10mg
$469.00
Ship with 5-10 days

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Ac-YVAD-CHO is a potent, specific, reversible inhibitor of caspase-1, completely blocking caspase-1 activity (99%) at the concentration of 1 μM [1]. 

Caspase-1 is an evolutionarily conserved enzyme that proteolytically cleaves other proteins, the activation of which results in the processing and release of cytokines, interleukin 1β (IL-1β) and interleukin 18 (IL-18), as well as pyroptosis, an immunogenic form of cell death [1]. 

In THP-1 cells treated with α-hemolysin, Ac-YVAD-CHO inhibited 99% of the activity induced by hemolysin at 1 μM [1]. In xanthine/xanthine-oxidase (X/XO)-treated G85R-expressing N2a cells, Ac-YVAD-CHO at 100 μM, protected G85R-expressing cells against X/XO-induced loss of cell viability, caspase-1 cleavage, and increased IL-1β secretion [2]. 

In toluene diisocyanate (TDI)-exposed mice, Ac-YVAD-CHO (1 mg/kg, i.n., q.d., for 7 consecutive days) effectively inhibited airway hyperresponsiveness (AHR), airway inflammation around the airways and epithelial goblet cell hyperplasia. In addition, Ac-YVAD-CHO robustly decreased the numbers of TH1/TH2 cells, and lowered levels of IL-18 and IL-1β [3]. 

References:

[1]. O'Brien M, Moehring D, Muñoz-Planillo R, et al. A bioluminescent caspase-1 activity assay rapidly monitors inflammasome activation in cells. Journal of Immunological Methods, 2017, 447: 1-13.

[2]. Pasinelli P, Borchelt D R, Houseweart M K, et al. Caspase-1 is activated in neural cells and tissue with amyotrophic lateral sclerosis-associated mutations in copper-zinc superoxide dismutase. Proceedings of the National Academy of Sciences of the United States of America. 1998, 95(26): 1576-15768.

[3]. Chen S, Yao L, Huang P, et al. Blockade of the NLRP3/caspase-1 axis ameliorates airway neutrophilic inflammation in a toluene diisocyanate induced murine asthma model. Toxicological Sciences, 2019, 170(2): 462-472.

Product Citation

Chemical Properties

Physical AppearanceA crystalline solid
StorageStore at -20°C
M.Wt492.5
Cas No.143313-51-3
FormulaC23H32N4O8
SolubilitySoluble in DMSO
Chemical Name(4S,7S,10S,13S)-13-formyl-4-(4-hydroxybenzyl)-7-isopropyl-10-methyl-2,5,8,11-tetraoxo-3,6,9,12-tetraazapentadecan-15-oic acid
SDFDownload SDF
Canonical SMILESCC(N([[email protected]](C(N([[email protected]](C(N([[email protected]](C(N([[email protected]](C([H])=O)CC(O)=O)[H])=O)C)[H])=O)C(C)C)[H])=O)CC1=CC=C(C=C1)O)[H])=O
Shipping ConditionShip with blue ice, or upon other requests.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.

Quality Control

Quality Control & MSDS

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Chemical structure

Ac-YVAD-CHO

Related Biological Data

Ac-YVAD-CHO