A 61603 hydrobromide
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
A 61603 hydrobromide is a potent and selective agonist of α1A-adrenoceptor .
α1A-adrenoceptor (α1A-AR) belongs to α1- adrenergic receptors, which include three subtypes α1A, α1B and α1D and play an important role in regulating cell growth and proliferation.
A 61603 hydrobromide is a potent and selective α1A-adrenoceptor agonist. A-61603 was 35-fold more potent at α1A-AR than at α1B-AR or α1D-AR. In fibroblast cells transfected with α1A-AR, A-61603 significantly stimulated phosphoinositide hydrolysis . A 61603 exhibited affinity for α1A-AR, α1B-AR and α1D-AR with pKi values of 8.05/7.52, 5.68 and 5.87, respectively. Also, A 61603 exhibited agonist activities for α1A-AR, α1B-AR and α1D-AR with pEC50 values of 8.24/7.66, 6.50 and 5.59, respectively . In neonatal rat ventricular myocytes, A61603 significantly increased the frequency of Ca2+ transients with EC50 value of 6.9 nM in a dose-dependent way . In mesenteric vascular bed isolated from mice, A61603 significantly increased perfusion pressure with 235-fold higher potency than phenylephrine, which suggested that α1A-AR plays an important role in the control of blood pressure .
. Knepper SM, Buckner SA, Brune ME, et al. A-61603, a potent alpha 1-adrenergic receptor agonist, selective for the alpha 1A receptor subtype. J Pharmacol Exp Ther, 1995, 274(1): 97-103.
. Meyer MD, Altenbach RJ, Hancock AA, et al. Synthesis and in vitro characterization of N-[5-(4,5-dihydro-1H-imidazol-2-yl)-2-hydroxy-5,6,7,8- tetrahydronaphthalen-1-yl]methanesulfonamide and its enantiomers: a novel selective alpha 1A receptor agonist. J Med Chem, 1996, 39(20): 4116-4119.
. Luo DL, Gao J, Fan LL, et al. Receptor subtype involved in alpha 1-adrenergic receptor-mediated Ca2+ signaling in cardiomyocytes. Acta Pharmacol Sin, 2007, 28(7): 968-974.
. Martínez-Salas SG1, Campos-Peralta JM, Pares-Hipolito J, et al. Alpha1A-adrenoceptors predominate in the control of blood pressure in mouse mesenteric vascular bed. Auton Autacoid Pharmacol, 2007, 27(3): 137-142.
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Solubility||Soluble in H2O|
|Chemical Name||(S)-N-(5-(4,5-dihydro-1H-imidazol-2-yl)-2-hydroxy-5,6,7,8-tetrahydronaphthalen-1-yl)methanesulfonamide hydrobromide|
|Canonical SMILES||OC1=C(C2=C(C=C1)[[email protected]@H](C3=NCCN3)CCC2)NS(=O)(C)=O.Br|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|