In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
8-Aminoadenine is an agonist of adenine receptor with Ki value of 0.0341 μM .
Adenine receptor is a G protein-coupled receptor activated by the nucleoside adenosine. There are four types of adenosine receptors: A1, A2A, A2B and A3. A1 and A2A receptors play an important role in the heart, regulating coronary blood flow and myocardial oxygen consumption. A2B and A3 receptors are involved in inflammation and immune responses.
8-Aminoadenine is an adenine receptor agonist with Ki values of 0.0341 and 6.51 μM for human and rat, respectively. In HEK293 cell membrane, 8-Aminoadenine was more potent than adenine (Ki = 0.0471 μM) and exhibited 190-fold more potent than the rat binding site. In 1321N1 astrocytoma cells expressing the rAde1R, 8-Aminoadenine (500 μM) inhibited isoproterenol-stimulated cAMP accumulation, which suggested that 8-Aminoadenine inhibited adenine uptake .
. Borrmann T, Abdelrahman A, Volpini R, et al. Structure-activity relationships of adenine and deazaadenine derivatives as ligands for adenine receptors, a new purinergic receptor family. J Med Chem, 2009, 52(19): 5974-5989.
|Physical Appearance||Off-white solid|
|Storage||Store at -20°C|
|Solubility||<15.01mg/ml in 2eq.HCl; <7.51mg/ml in DMSO|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|