Home >> 740 Y-P
Related Products
740 Y-P PI 3-kinase activator,cell permeable

Catalog No.B5246
Size Price Stock Qty
1mg
$100.00
In stock
5mg
$250.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

      

Sample solution is provided at 25 µL, 10mM.

Product Citations

1. Wang X, Qi X, et al. "Autophagy Benefits the Replication of Egg Drop Syndrome Virus in Duck Embryo Fibroblasts." Front Microbiol. 2018 May 29;9:1091. PMID:29896171
2. Yang C, Hou A, et al. "Kanglaite reverses multidrug resistance of HCC by inducing apoptosis and cell cycle arrest via PI3K/AKT pathway." Onco Targets Ther. 2018 Feb 26;11:983-996. PMID:29520149
3. Shen Y, Wen L, et al. "Dihydrodiosgenin protects against experimental acute pancreatitis and associated lung injury through mitochondrial protection and PI3Kγ/Akt inhibition." Br J Pharmacol. 2018 May;175(10):1621-1636. PMID:29457828
4. Zhang X, Zhao F, et al. "PDGF-mediated PI3K/AKT/β-catenin signaling regulates gap junctions in corpus cavernosum smooth muscle cells." Exp Cell Res. 2017 Nov 22. pii: S0014-4827(17)30627-4. PMID:29174980

Quality Control

Chemical structure

740 Y-P

Related Biological Data

740 Y-P

Related Biological Data

740 Y-P

Related Biological Data

740 Y-P

Related Biological Data

740 Y-P

Protocol

Cell experiment [1]:

Cell lines

Human melanoma MNT-1 cells

Preparation method

Limited solubility. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reaction Conditions

24 h

Applications

740 Y-P induces the generation of M6PR-positive puncta which is similar to the sucrose-induced vacuoles. 740 Y-P also prominently reduces the number of M6PR-positive vacuoles triggers by the sucrose treatment.

References:

1. Bin BH, Bhin J, Yang SH et al. Hyperosmotic stress reduces melanin production by altering melanosome formation. PLoS One. 2014 Aug 29;9(8):e105965.

740 Y-P Dilution Calculator

Concentration (start)
x
Volume (start)
=
Concentration (final)
x
Volume (final)
 
 
 
C1
V1
C2
V2

calculate

740 Y-P Molarity Calculator

Mass
=
Concentration
x
Volume
x
MW*
 
 
 
g/mol

calculate

Chemical Properties

Cas No. 1236188-16-1 SDF N/A
Synonyms N/A
Chemical Name N/A
Canonical SMILES N/A
Formula C141H222N43O39PS3 M.Wt 3270.72
Solubility >163.5mg/mL in DMSO Storage Desiccate at -20°C
Shipping Condition Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Background

740 Y-P is an activator of PI3K with concentration of 20 μM [1].
PI3K (phosphoinositide 3-kinases, PI3Ks) is an enzyme and plays an important role in the fundamental cellular processes, such as vesicular trafficking, cell degranulation, cell migration, and glucose transporter. It has been reported that over-expression of PI3K was correlated with a variety kind of cancers [2].
740 Y-P is a potent PI3K activator and plays an important role in PI3K/AKT signaling pathway. When tested with human melanoma MNT-1 cells, 20 μM 740 Y-P for 24 hours treatment significantly reduced the number of M6PR-positive vacuoles induced by sucrose via activating PI3K [1]. In cerebellar granule cells in the circumstance of serum deprivation, 740 Y-P treatments reduced the cell death rate via binding to p85 which was correlated with PI 3-kinase-dependent phosphorylation of Akt process [3].
References:
[1].    Bin, B.H., et al., Hyperosmotic stress reduces melanin production by altering melanosome formation. PLoS One, 2014. 9(8): p. e105965.
[2].    Kong, B., et al., A subset of metastatic pancreatic ductal adenocarcinomas depends quantitatively on oncogenic Kras/Mek/Erk-induced hyperactive mTOR signalling. Gut, 2015.
[3].    Williams, E.J. and P. Doherty, Evidence for and against a pivotal role of PI 3-kinase in a neuronal cell survival pathway. Mol Cell Neurosci, 1999. 13(4): p. 272-80.