Toggle Nav

740 Y-P

In stock
Catalog No.
B5246
PI 3-kinase activator,cell permeable
Grouped product items
SizePriceStock Qty
1mg
$100.00
In stock
5mg
$250.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

740 Y-P is an activator of PI3K with concentration of 20 μM [1].
PI3K (phosphoinositide 3-kinases, PI3Ks) is an enzyme and plays an important role in the fundamental cellular processes, such as vesicular trafficking, cell degranulation, cell migration, and glucose transporter. It has been reported that over-expression of PI3K was correlated with a variety kind of cancers [2].
740 Y-P is a potent PI3K activator and plays an important role in PI3K/AKT signaling pathway. When tested with human melanoma MNT-1 cells, 20 μM 740 Y-P for 24 hours treatment significantly reduced the number of M6PR-positive vacuoles induced by sucrose via activating PI3K [1]. In cerebellar granule cells in the circumstance of serum deprivation, 740 Y-P treatments reduced the cell death rate via binding to p85 which was correlated with PI 3-kinase-dependent phosphorylation of Akt process [3].
References:
[1].    Bin, B.H., et al., Hyperosmotic stress reduces melanin production by altering melanosome formation. PLoS One, 2014. 9(8): p. e105965.
[2].    Kong, B., et al., A subset of metastatic pancreatic ductal adenocarcinomas depends quantitatively on oncogenic Kras/Mek/Erk-induced hyperactive mTOR signalling. Gut, 2015.
[3].    Williams, E.J. and P. Doherty, Evidence for and against a pivotal role of PI 3-kinase in a neuronal cell survival pathway. Mol Cell Neurosci, 1999. 13(4): p. 272-80.

Product Citation

Chemical Properties

Physical AppearanceA solid
StorageDesiccate at -20°C
M.Wt3270.72
Cas No.1236188-16-1
FormulaC141H222N43O39PS3
Solubility≥163.536mg/mL in DMSO
Shipping ConditionEvaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Protocol

Cell experiment [1]:

Cell lines

Human melanoma MNT-1 cells

Preparation method

Limited solubility. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reaction Conditions

24 h

Applications

740 Y-P induces the generation of M6PR-positive puncta which is similar to the sucrose-induced vacuoles. 740 Y-P also prominently reduces the number of M6PR-positive vacuoles triggers by the sucrose treatment.

References:

1. Bin BH, Bhin J, Yang SH et al. Hyperosmotic stress reduces melanin production by altering melanosome formation. PLoS One. 2014 Aug 29;9(8):e105965.

Quality Control

Chemical structure

740 Y-P

Related Biological Data

740 Y-P

Related Biological Data

740 Y-P

Related Biological Data

740 Y-P

Related Biological Data

740 Y-P

Related Biological Data

740 Y-P