In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
7α,25-dihydroxy Cholesterol (7α,25-DHC, 7α,25-OHC) is a potent and selective GPR183 agonist .
Epstein-Barr virus (EBV)-induced gene 2 (EBI2, also known as GPR183) is an orphan G protein-coupled receptor that is highly expressed in spleen and up-regulated upon Epstein–Barr-virus infection. GPR183 is required for humoral immune responses and polymorphisms in the receptor is associated with inflammatory autoimmune diseases .
7α,25-dihydroxy Cholesterol is a potent and selective GPR183 (EBI2) agonist and most potent EBI2 ligand with EC50 and Kd values of 140 pM and 450 pM, respectively. In a competition binding study, 7α,25-OHC competed for binding with IC50 value of 242 pM. In EBI2-expressing cells, 7α,25-OHC dose-dependently inhibited forskolin-induced cAMP with IC50 value of 2 nM. 7α,25-OHC was probably the endogenous ligand for EBI2. In EBI2-expressing mouse B and T cells, 7α,25-dihydroxy Cholesterol stimulated cell migration with IC50 value of around 500 pM, but had no effect on EBI2-deficient cells .
In EBI2-deficient mice, unseparated splenocytes, B cells and T cells were completely unresponsive to 7α,25-OHC stimulation . Mice deficient in Ch25h, a key enzyme for the generation of 7α, 25-OHC, failed to position activated B cells within the spleen to the outer follicle .
. Liu C, Yang XV, Wu J, et al. Oxysterols direct B-cell migration through EBI2. Nature. 2011 Jul 27;475(7357):519-23.
. Hannedouche S, Zhang J, Yi T, et al. Oxysterols direct immune cell migration via EBI2. Nature. 2011 Jul 27;475(7357):524-7.
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Solubility||≤2mg/ml in ethanol;0.1mg/ml in DMSO;20mg/ml in dimethyl formamide|
|Canonical SMILES||O[[email protected]@H]1C=C2C[[email protected]@H](O)CC[[email protected]]2(C)[[email protected]]3([H])[[email protected]]1([H])[[email protected]@](CC[[email protected]]4([H])[[email protected]](C)CCCC(C)(C)O)([H])[[email protected]]4(C)CC3|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|