(5Z)-7-Oxozeaenol
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
(5Z)-7-Oxozeaenol is a natural resorcylic lactone derived from fungi that selectively inhibits purified TAK-1 (IC50 = 8.1 nM) without significantly affecting the kinase activities of other MAPKKK family members.
In cells, (5Z)-7-Oxozeaenol is reported to irreversibly block IL-1-induced activation of TAK-1, NF-κB, and JNK/p38 with IC50 values of 65-83 nM.
(5Z)-7-Oxozeaenol has also been shown to inhibit IL-1-induced COX-2 production in cells and to relieve ear swelling induced by picryl chloride in a mouse model of COX-2-mediated inflammation.
Reference:
[1]. Ninomiya-Tsuji J, Kajino T, Ono K, et al. A resorcylic acid lactone, 5Z-7-oxozeaenol, prevents inflammation by inhibiting the catalytic activity of TAK1 MAPK kinase kinase. J. Biol. Chem, 2003, 278(20): 18485-18490.
| Physical Appearance | White solid |
| Storage | Desiccate at -20°C |
| M.Wt | 362.37 |
| Cas No. | 253863-19-3 |
| Formula | C19H22O7 |
| Solubility | <9.06mg/ml in DMSO;insoluble in ethanol |
| Chemical Name | (3S,5Z,8S,9S,11E)-8,9,16-trihydroxy-14-methoxy-3-methyl-3,4,9,10-tetrahydro-1H-benzo[c][1]oxacyclotetradecine-1,7(8H)-dione |
| SDF | Download SDF |
| Canonical SMILES | O[[email protected]@H](C/C=C/C(C=C1OC)=C(C(O)=C1)C(O[[email protected]@H](C)C/C=C\C2=O)=O)[[email protected]@H]2O |
| Shipping Condition | Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request. |
| General tips | For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months. |
| Cell experiment:[1] | |
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Cell lines |
Mouse embryonic fibroblast cells |
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Reaction Conditions |
500 nM (5Z)-7-oxozeaenol for 17.5 h incubation |
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Applications |
In cell culture, (5Z)-7-oxozeaenol blocked interleukin-1-induced activation of TAK1, JNK/p38 MAPK, IκB kinases, and NF-κB, resulting in inhibition of cyclooxgenase-2 production. |
| Animal experiment:[1] | |
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Animal models |
Mice repeatedly sensitized by picryl chloride (PC) and subsequently challenged with PC |
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Dosage form |
10 μl of (5Z)-7-oxozeaenol (1 mg/ml) Painted on each ear 2 h before and 4 h after the second PC challenge (total of twice) or every hour three times before and 4 h after the second PC challenge (total of four times) |
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Applications |
When (5Z)-7-oxozeaenol was administrated to the PC-challenged ear, ear swelling was reduced by up to 50% of that of the control ear treated with vehicle. Thus, (5Z)-7-oxozeaenol was able to prevent inflammation by inhibiting the catalytic activity of TAK1 MAPKKK. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Ninomiya-Tsuji J, Kajino T, Ono K, et al. A resorcylic acid lactone, 5Z-7-oxozeaenol, prevents inflammation by inhibiting the catalytic activity of TAK1 MAPK kinase kinase. Journal of Biological Chemistry, 2003, 278(20): 18485-18490. |
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Quality Control & MSDS
- View current batch:
-
Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
