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5-Ethynyl-2'-deoxyuridine (5-EdU)

Catalog No.
B8337
Nucleoside analog of thymidine for fast and sensitive detection of DNA synthesis
Grouped product items
SizePriceStock Qty
100mg
$100.00
In stock
1g
$450.00
In stock

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Email: [email protected]

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Description

5-Ethynyl-2'-deoxyuridine (5-EdU) is a thymidine analog that can be incorporated into replicating DNA for detection of cell proliferation. 5-EdU can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle through click chemistry. Click chemistry is a method of covalently coupling an azide with an alkyne. 5-EdU has an alkyne handle that can be detected using azide-containing fluorescent probes through a highly efficient click chemistry reaction [1][2]. In contrast to the use of BrdU, the new method does not require sample fixation or DNA denaturation. 5-EdU-labelled cells may be isolated to determine the transcript of cells, from neural cancer or tissues that have undergone division in vitro and in vivo[3].

References:

[1] Zeng C, Pan F, et al. Evaluation of 5-ethynyl-2'-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system. Brain Res. 2010 Mar 10;1319:21-32.

[2] Cavanagh BL, Walker T, et al. Thymidine analogues for tracking DNA synthesis. Molecules. 2011 Sep 15;16(9):7980-93.

[3] Endaya BB, Lam PY, et al. Transcriptional profiling of dividing tumor cells detects intratumor heterogeneity linked to cell proliferation in a brain tumor model. Mol Oncol. 2016 Jan;10(1):126-37.

Chemical Properties

StorageStore at -20°C
M.Wt252.22
Cas No.61135-33-9
FormulaC11H12N2O5
SolubilitySoluble in DMSO
Chemical Name5-ethynyl-1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)pyrimidine-2,4(1H,3H)-dione
SDFDownload SDF
Canonical SMILESO=C1C(C#C)=CN([[email protected]@H]2O[[email protected]](CO)[[email protected]@H](O)C2)C(N1)=O
Shipping ConditionEvaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

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