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5-Ethynyl-2'-deoxyuridine (5-EdU)

Catalog No.
Nucleoside analog of thymidine for fast and sensitive detection of DNA synthesis
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5-Ethynyl-2'-deoxyuridine (5-EdU) is a thymidine analog that can be incorporated into replicating DNA for detection of cell proliferation. 5-EdU can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle through click chemistry. Click chemistry is a method of covalently coupling an azide with an alkyne. 5-EdU has an alkyne handle that can be detected using azide-containing fluorescent probes through a highly efficient click chemistry reaction [1][2]. In contrast to the use of BrdU, the new method does not require sample fixation or DNA denaturation. 5-EdU-labelled cells may be isolated to determine the transcript of cells, from neural cancer or tissues that have undergone division in vitro and in vivo[3].


[1] Zeng C, Pan F, et al. Evaluation of 5-ethynyl-2'-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system. Brain Res. 2010 Mar 10;1319:21-32.

[2] Cavanagh BL, Walker T, et al. Thymidine analogues for tracking DNA synthesis. Molecules. 2011 Sep 15;16(9):7980-93.

[3] Endaya BB, Lam PY, et al. Transcriptional profiling of dividing tumor cells detects intratumor heterogeneity linked to cell proliferation in a brain tumor model. Mol Oncol. 2016 Jan;10(1):126-37.

Chemical Properties

Physical AppearanceSolution
StorageStore at -20°C
Cas No.61135-33-9
Solubility≥25.2 mg/mL in DMSO; insoluble in EtOH; ≥3.16 mg/mL in H2O with gentle warming and ultrasonic
Chemical Name5-ethynyl-1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)pyrimidine-2,4(1H,3H)-dione
SDFDownload SDF
Canonical SMILESO=C1C(C#C)=CN([[email protected]@H]2O[[email protected]](CO)[[email protected]@H](O)C2)C(N1)=O
Shipping ConditionEvaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

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