In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
2-chloro-3-Deazaadenosine, a stable analog of adenosine, is an agonist for adenosine receptors .
Adenosine receptors are members of G-protein-coupled receptors (GPCRs). Extracellular adenosine acts as a local modulator with a generally cytoprotective function in the body. Extracellular adenosine has been implicated in increasing the ratio of oxygen supply to demand, protecting against ischaemic damage by cell conditioning, triggering anti-inflammatory responses and the promotion of angiogenesis .
In vitro: The Ki values of 2-chloro-3-Deazaadenosine for A1, A2A, A2B, and A3 receptors were 0.3, 0.08, 25.5, and 1.9 μM, respectively . 2-chloroadenosine (25 μM) increased activity of platelet adenylate cyclase to about 150–160% of the control value. Higher concentrations of 2-chloroadenosine showed less effect above 100 μM .
In vivo: In the rat with delayed ischaemic damage, Iterative focal injections of 2-chloroadenosine protected against selective hippocampal CA1 loss .
 Linden J, Thai T, Figler H, et al. Characterization of Human A2B Adenosine Receptors: Radioligand Binding, Western Blotting, and Coupling to Gqin Human Embryonic Kidney 293 Cells and HMC-1 Mast Cells[J]. Molecular Pharmacology, 1999, 56(4): 705-713.
 Jacobson K A, Gao Z G. Adenosine receptors as therapeutic targets[J]. Nature Reviews Drug Discovery, 2006, 5(3): 247-264.
 Haslam R J, Lynham J A. Activation and inhibition of blood platelet adenylate cyclase by adenosine or by 2-chloroadenosine[J]. Life Sciences, 1972, 11(23): 1143-1154.
 Evans M C, Swan J H, Meldrum B S. An adenosine analogue, 2-chloroadenosine, protects against long term development of ischaemic cell loss in the rat hippocampus[J]. Neuroscience letters, 1987, 83(3): 287-292.
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Synonyms||6-Amino-2-chloropurine riboside,2-CADO,NSC 158900|
|Solubility||≤0.25mg/ml in ethanol;2mg/ml in DMSO;2mg/ml in dimethyl formamide|
|Canonical SMILES||O[[email protected]]1[[email protected]](N2C=NC3=C2C=C(Cl)N=C3N)O[[email protected]](CO)[[email protected]]1O|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|