X-Gal
Catalog No.
A2539
Substrate for β-galactosidase,used in blue/white screening
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X-Gal (CAS 7240-90-6) is a chromogenic substrate widely used for detecting β-galactosidase enzyme activity. Upon hydrolysis by β-galactosidase, X-Gal releases galactose and a chromophore, 4-chloro-3-bromo-indigo, which precipitates as an insoluble blue product. This property is utilized in molecular biology for blue-white screening to differentiate recombinant from non-recombinant bacterial clones. Recombinant plasmids disrupt the α-peptide of the lacZ gene, preventing complementation and resulting in white colonies. It is routinely employed alongside IPTG in genetic cloning experiments involving lacZ systems.
- 1. Stephanie A Azzopardi, Hsiu-Yi Lu, et al. "Role of iRhom2 in Olfaction: Implications for Odorant Receptor Regulation and Activity-Dependent Adaptation." Int J Mol Sci. 2024 May 31;25(11):6079. PMID: 38892263
- 2. Morgan E. Mouchka, Dillon M. Dorsey, et al. "Viral host-range evolvability changes in response to fluctuating selection." bioRxiv. 2019 September 18
| Physical Appearance | A crystalline solid |
| Storage | Store at -20°C |
| M.Wt | 408.63 |
| Cas No. | 7240-90-6 |
| Formula | C14H15BrCINO6 |
| Solubility | insoluble in H2O; ≥109.4 mg/mL in DMSO; ≥3.7 mg/mL in EtOH with gentle warming and ultrasonic |
| Chemical Name | (2S,3R,4S,5R,6R)-2-[(5-bromo-4-chloro-1H-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol |
| SDF | Download SDF |
| Canonical SMILES | C1=CC(=C(C2=C1NC=C2OC3C(C(C(C(O3)CO)O)O)O)Cl)Br |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Chemical structure
