In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
UCN-02 is a derivative of staurosporine and a stereoisomer of UCN-01, which has been first isolated from a high staurosporine-producing Streptomyces culture as a minor co-metabolite . UCN-02 is protein kinase C inhibitor .
Protein kinase C (PKC), which can phosphorylate serine and threonine, is a family of protein kinase enzymes involved in regulating the function of other proteins. Protein kinase C plays an important role in several signal transduction cascades. Protein kinase C has been implicated in modulating membrane structure events, mediating immune responses, regulating transcription, learning and memory, and regulating cell growth .
UCN-02 inhibited protein kinase C with a slightly reduced potency than UCN-01. The IC50 values were 62 and 4.1 nM for UNC-02 and UCN-01, respectively. UCN-02 was cytotoxic to the growth of HeLa S3 cells.
 Takahashi, I. ,Saitoh, Y.,Yoshida, M., et al. UCN-01 and UCN-02, new selective inhibitors of protein kinase C. II. Purification, physico-chemical properties, structural determination and biological activities. J.Antibiot.(Tokyo) 42(4), 571-576 (1989).
 Newton A C. Protein kinase C: structure, function, and regulation[J]. Journal of Biological Chemistry, 1995, 270(48): 28495-28498.
|Physical Appearance||A solution in ethanol. To change the solvent, simply evaporate the ethanol under a gentle stream of nitrogen and immediately add the solvent of choice.|
|Storage||Store at -20°C|
|Solubility||Soluble in DMSO|
|Canonical SMILES||[H][[email protected]]12N(C3=C(N([[email protected]](O2)(C)[[email protected]](OC)[[email protected]](NC)C1)C4=C5C=CC=C4)C5=C([[email protected]](O)NC6=O)C6=C37)C8=C7C=CC=C8|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|