Toggle Nav
Close
  • Menu
  • Setting

Tunicamycin

Catalog No.
B7417
antibiotic,inhibits GlcNAc phosphotransferase (GPT)
Grouped product items
SizePriceStock Qty
1mg
$105.00
In stock
5mg
$172.00
In stock
10mg
$307.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Tunicamycin (CAS 11089-65-9) is an antibiotic commonly utilized as an inhibitor of protein N-glycosylation. It acts by blocking the initial transfer reaction between UDP-N-acetylglucosamine and polyisoprenol phosphate, thus preventing formation of dolichol pyrophosphate N-acetylglucosamine intermediates essential for N-linked glycoprotein synthesis. This interference induces endoplasmic reticulum (ER) stress within affected cells. Experimentally, tunicamycin effectively suppresses inflammatory responses in RAW264.7 macrophages stimulated by lipopolysaccharide, reducing expression and release of inflammatory mediators such as COX-2 and iNOS. Additionally, it modulates stress-related gene expression in murine intestinal and hepatic tissues, making it valuable for studies of ER stress, glycosylation pathways, and inflammation.

Product Citation

Chemical Properties

Physical AppearanceA crystalline solid
StorageStore at -20°C
M.Wt844.95
Cas No.11089-65-9
FormulaC39H64N4O16 (tunicamycin C, n=10)
Solubility≥25 mg/mL in DMSO
SDFDownload SDF
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment [1]:

Cell lines

RAW264.7 cells

Preparation method

The solubility of this compound in DMSO is >25 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

48 h, 0.5 μg/mL

Applications

In RAW264.7 cells, tunicamycin significantly reduced LPS-induced nitrite release/production and attenuated the expression of mRNAs and hence proteins of COX-2 and iNOS. Tunicamycin at a concentration of 0.5 μg/mL did not have any effect on cell survival/proliferation, but at 48 h tunicamycin provided protection against activation-induced macrophage cell death. In a concentration-dependent manner, tunicamycin reduced COX-2 and iNOS protein expression in response to LPS and induced a concurrent increase in 78-kDa glucose-regulated protein (GRP78), an ER (endoplasmic reticulum) chaperone.

Animal models

C57BL/6J Nrf2 (+/+; wildtype) and C57BL/6J/Nrf2(-/-; knockout) mice

Dosage form

Oral gavage and only once for 3 h with 2 mg/kg tunicamycin (dissolved in 50% PEG 400 aqueous solution).

Application

In the small intestine of wild-type mice, expression levels of 1291 probes were elevated or of 1370 probes were suppressed >2 fold by tunicamycin. In the small intestine of Nrf2(-/-) mice, tunicamycin inhibited 2024 probes and induced 3471 probes by >2 fold. Compared with results of small intestine samples, in wild-type mice liver, less well-defined genes were either suppressed (943) or elevated (750) >2 fold by tunicamycin; whereas in Nrf2(-/-) mice liver, 3170 genes were inhibited or 39 well-defined genes were induced.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1] Song-YiKim, Ji-SunHwang and Inn-OcHan. Tunicamycin inhibits Toll-like receptor-activated inflammation in RAW264.7 cells by suppression of NF-κB and c-Jun activity via a mechanism that is independent of ER-stress and N-glycosylation. European Journal of Pharmacology, 2013, 721: 294-300.

[2] Sujit Nair, Changjiang Xu, Guoxiang Shen, et al. Toxicogenomics of Endoplasmic Reticulum stress inducer Tunicamycin in the Small Intestine and Liver of Nrf2 Knockout and C57BL/6J Mice. Toxicol Lett., 2007, 168(1):21-39.

Quality Control

Quality Control & MSDS

View current batch:

Chemical structure

Tunicamycin

Related Biological Data

Tunicamycin

Related Biological Data

Tunicamycin

Related Biological Data

Tunicamycin

Related Biological Data

Tunicamycin

Related Biological Data

Tunicamycin

Related Biological Data

Tunicamycin

Related Biological Data

Tunicamycin