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TNF-alpha, recombinant rat protein

Catalog No.
P1003
TNF-α, expressed in E. coli, suitable for cell culture.
Grouped product items
SizePriceStock Qty
10ug
$178.00
Ship with 5-10 days
50ug
$429.00
Ship with 5-10 days
1mg
$3,762.00
Ship with 5-10 days

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Email: [email protected]

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Background

Rat tumor Necrosis Factor-α (TNF-α) is a potent cytokine expressed as a transmembrane protein with 235 aa that is cleaved to the soluble monomer by TNF-α converting enzyme (TACE) and forms stable homotrimers. TNF-α is expressed in many cells and plays an important role in inflammation, viral replication, tumorigenesis, apoptosis, cell proliferation, differentiation, coagulation and lipid metabolism. TNF-α is mainly produced by macrophages when challenged with viruses or bacteria. TNF-α is cytotoxic for many tumor cells lines but induced activation, differentiation or proliferation in normal cells.

Biological Activity

Determined by the cytolysis of murine L929 cells in the presence of actinomycin D.
ED50 < 0.05 ng/ml.          
Specific activity > 2*107 units/mg.          

Quality Control

Quality Control & DataSheet

View current batch:
    Purity: ≥95%, by SDS-PAGE and HPLC.
    Endotoxin Level:<0.1 ng/μg

Related Biological Data

TNF-alpha

Related Biological Data

TNF-alpha
 

Description

Gene ID: 24835 Accession #: P16599.1
Alternate Names:TNFA, TNF alpha; TNF-a; cachectin; tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha.
Source: Escherichia Coli. M.Wt: 17 kDa
Appearance: Lyophilized powder Storage: Store at -20°C
Formulation:Sterile filtered and lyophilized from 1× PBS.
Solubility:Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4℃ for 1 week or -20℃ for future use.
Shipping Condition: Gel pack. Handling: Centrifuge the vial prior to opening.
Usage:For Research Use Only! Not to be used in humans.