TGF-beta1, human recombinant protein (CHO cells)

Gene ID

7040

Accession #

P01137

Alternate Names

TGFB1, Human TGF-beta1, TGF-beta1, TGF beta1, TGFbeta1, h-TGF-beta1, rh-TGF-beta1, recombinant human TGF-beta1, recombinant TGF-beta, TGF.

Source

Chinese Hamster Ovary cell line, CHO

M.Wt

Apparent molecular mass of 24 kDa in SDS-PAGE under non-reducing conditions, 12 kDa under reducing conditions, a disulfide-linked homodimer of two 112 amino acid glycosylated polypeptide chains.

AA Sequence

Ala279-Ser390

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Formulation

Lyophilized from 0.2 µm filtered concentrated solution in 35 % Acetonitrile and 0.1 % TFA.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile 4 mM HCl to a concentration of 0.1 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriately buffered solutions.

Biological Activity

Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. The ED₅₀ for this effect is 0.04-0.2 ng/mL. The specific activity of rHuTGF-β1 is approximately 2.5 × 10⁴ U/μg, which is calibrated against human TGF-β1 Standard.

Shipping Condition

Gel pack.

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans.