TGF-beta1, human recombinant protein (CHO cells)
TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some non‑redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation. TGF‑ beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins. Mature human TGF‑ beta 1 shares 100% aa identity with pig, dog and cow TGF‑ beta 1, and 99 % aa identity with mouse, rat and horse TGF‑beta 1.
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Gene ID |
7040 |
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Accession # |
P01137 |
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Alternate Names |
TGFB1, Human TGF-beta1, TGF-beta1, TGF beta1, TGFbeta1, h-TGF-beta1, rh-TGF-beta1, recombinant human TGF-beta1, recombinant TGF-beta, TGF. |
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Source |
Chinese Hamster Ovary cell line, CHO |
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M.Wt |
Apparent molecular mass of 24 kDa in SDS-PAGE under non-reducing conditions, 12 kDa under reducing conditions, a disulfide-linked homodimer of two 112 amino acid glycosylated polypeptide chains. |
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AA Sequence |
Ala279-Ser390 |
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Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
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Formulation |
Lyophilized from 0.2 µm filtered concentrated solution in 35 % Acetonitrile and 0.1 % TFA. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile 4 mM HCl to a concentration of 0.1 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriately buffered solutions. |
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Biological Activity |
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. The ED50 for this effect is 0.04-0.2 ng/mL. The specific activity of rHuTGF-β1 is approximately 2.5 × 104 U/μg, which is calibrated against human TGF-β1 Standard. |
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Shipping Condition |
Gel pack. |
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Handling |
Centrifuge the vial prior to opening. |
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Usage |
For Research Use Only! Not to be used in humans. |
- 1. Yan Hu, Lulu Yang, et al. "m6A reader IGF2BP1 facilitates macrophage glycolytic metabolism and fibrotic phenotype by stabilizing THBS1 mRNA to promote pulmonary fibrosis." Cell Mol Life Sci. 2025 Apr 12;82(1):157 PMID: 40220148
- 2. Chunfeng Xie, Jianyun Zhu, et al. "TAp63α Is Involved in Tobacco Smoke-Induced Lung Cancer EMT and the Anti-cancer Activity of Curcumin via miR-19 Transcriptional Suppression." Front Cell Dev Biol. 2021 Mar 4;9:645402. PMID:33748140
Quality Control & DataSheet
- View current batch:
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Purity > 97 % by SDS-PAGE analyses.
- Datasheet
Endotoxin Level: Less than 0.1 EU/µg of rHuTGF-β1 as determined by LAL method.
Related Biological Data
Related Biological Data
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