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Sulforaphane

Catalog No.
C4733
inducer of chemopreventative enzymes via Keap1-Nrf2 signaling
Grouped product items
SizePriceStock Qty
10mg
$60.00
In stock
25mg
$135.00
In stock
50mg
$250.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

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Background

Sulforaphane (CAS 4478-93-7) is a naturally occurring isothiocyanate abundantly found in vegetables, especially broccoli. It acts primarily through activation of the Keap1-Nrf2 signaling pathway, enhancing cellular defense against oxidative and electrophilic stress. Sulforaphane has demonstrated antiproliferative activity in vitro, as shown by dose-dependent cell cycle arrest at G2/M and apoptosis induction in HT29 human colon carcinoma cells. Such effects involve elevated cyclin A and B1 levels, increased pro-apoptotic Bax expression, mitochondrial cytochrome c release, and poly(ADP-ribose) polymerase cleavage. These properties make sulforaphane relevant for investigating cancer chemoprevention and cellular stress responses.

Product Citation

Chemical Properties

StorageStore at -20°C, Light exposure should be avoided
M.Wt177.3
Cas No.4478-93-7
FormulaC6H11NOS2
SynonymsSFN
Solubility≥51.6 mg/mL in H2O; ≥58.2 mg/mL in EtOH; ≥67.6 mg/mL in DMSO
Chemical Name1-isothiocyanato-4-(methylsulfinyl)-butane
SDFDownload SDF
Canonical SMILESCS(CCCCN=C=S)=O
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment:[1]

Cell lines

HT29 human colon cancer cells

Reaction Conditions

0 ~ 30 μM sulforaphane for 48 h incubation

Applications

Sulforaphane resulted in a net decrease in the total number of cells and an accumulation of cells floating in the culture medium. The inhibition of growth and the effect on the adherence were already important at 15 μM. Sulforaphane at 15 μM was able to inhibit cell growth and induce cell death. Cell mortality was already 75% at 24 h and almost total at 96 h.

Animal experiment:[2]

Animal models

Female Sprague-Dawley rats, 40 days of age

Dosage form

75 or 150 μmol

Once daily by gavage for 5 days

Applications

When sulforaphane was administered by gavage (75 or 150 μmol per day for 5 days) around the time of exposure to the 9,10-dimethyl-1,2-benzanthracene carcinogen, the incidence, multiplicity, and weight of mammary tumors were significantly reduced, and their development was delayed. Sulforaphane was able to protect against carcinogenesis.

Note

The technical data provided above is for reference only.

References:

1. Gamet-Payrastre L, Li P, Lumeau S, et al. Sulforaphane, a naturally occurring isothiocyanate, induces cell cycle arrest and apoptosis in HT29 human colon cancer cells. Cancer Research, 2000, 60(5): 1426-1433.

2. Zhang Y, Kensler TW, Cho CG, et al. Anticarcinogenic activities of sulforaphane and structurally related synthetic norbornyl isothiocyanates. Proceedings of the National Academy of Sciences of the United States of America, 1994, 91(8): 3147-3150.

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