In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Solamargine, apoisonouschemical compound, occurs in plants of theSolanaceaefamily, such aspotatoes,tomatoes, andeggplants.Solamargine has been also isolated fromSolanum nigrumfungalendophyteAspergillus flavus.
In vitro: Solamargine significantly inhibited migration and invasion of HepG2 cells by down-regulation of MMP-2 and MMP-9 expression and activity. Solamargine reduced HepG2 cell viability in a concentration-dependent manner. In HepG2 cells, solamargine (7.5 μM) decreased cell viability by less than 20%.Solamargineat the highest dose decreased cell migration and invasion by more than 70% and 72%, respectively.Solamarginealso reduced expression and function of MMP-2 and MMP-9 proteins .In lung cancer cells, Solamargine inhibited proliferation and induced apoptosis through p38 MAPK-mediated suppression of phosphorylation and protein expression of Stat3, followed by inducing Stat3 downstream effector p21. In non-small-cell lung cancer (NSCLC) cells, solamargine inhibited growth and induced apoptosis of in a time- and dose-dependent manner.Solamargine also increased phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK) in a time-dependent fashion .In SMMC-7721 cells, solamargine exerted potential anticancer activity through activation of caspase-3 and regulation of the cell cycle progression to induce apoptosis and inhibit hepatoma cells proliferation .In WM115 and WM239 cells, solamargine rapidly, selectively and effectively inhibited the growth of cells WM239 and WM115. Solamarginealso showed high efficacy against the vertical growth phase melanoma cells .
. Al Chami L, Méndez R, Chataing B, et al. Toxicological effects of α‐solamargine in experimental animals[J]. Phytotherapy Research, 2003, 17(3): 254-258.
. El‐Hawary S S, Mohammed R, AbouZid S F, et al. Solamargine production by a fungal endophyte of Solanum nigrum[J]. Journal of applied microbiology, 2016.
. Sani I K, Marashi S H, Kalalinia F. Solamargine inhibits migration and invasion of human hepatocellular carcinoma cells through down-regulation of matrix metalloproteinases 2 and 9 expression and activity[J]. Toxicology in Vitro, 2015, 29(5): 893-900.
. Aggarwal B B, Sethi G, Ahn K S, et al. Targeting Signal‐Transducer‐and‐Activator‐of‐Transcription‐3 for Prevention and Therapy of Cancer[J]. Annals of the New York Academy of Sciences, 2006, 1091(1): 151-169.
. Ding X, Zhu F S, Li M, et al. Induction of apoptosis in human hepatoma SMMC-7721 cells by solamargine from Solanum nigrumL[J]. Journal of ethnopharmacology, 2012, 139(2): 599-604.
. Al Sinani S S, Eltayeb E A, Coomber B L, et al. Solamargine triggers cellular necrosis selectively in different types of human melanoma cancer cells through extrinsic lysosomal mitochondrial death pathway[J]. Cancer cell international, 2016, 16(1): 1.
|Storage||Store at -20°C|
|Solubility||≥86.8 mg/mL in DMSO; insoluble in H2O; ≥36.6 mg/mL in EtOH|
|Canonical SMILES||C[[email protected]]1CN[[email protected]@]2(O[[email protected]]3(C[[email protected]]4([[email protected]@]5(CC=C6C[[email protected]@H](O[[email protected]]7([H])O[[email protected]](CO)[[email protected]@H](O[[email protected]@]8([H])[[email protected]](O)[[email protected]](O)[[email protected]@H](O)[[email protected]](C)O8)[[email protected]](O)[[email protected]]7O[[email protected]@]9([H])[[email protected]](O)[[email protected]](O)[[email protected]@H](O)[[email protected]](C)O9)CC[[email protected]@]6([[email protected]]5(CC[[email protected]@]4([[email protected]]3([[email protected]@H]2C)[H])C)[H])C)[H])[H])[H])CC1|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|