In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
SMER 28 is a new small-molecule enhancer of the cytostatic effects. The target of rapamycin proteins has been reported to be able to regulate various cellular processes including autophagy, which may play a protective role in some neurodegenerative and infectious diseases.
In vitro: SMER 28 independently induced autophagy of rapamycin in mammalian cells, enhancing the clearance of autophagy substrates such as A53T a-synuclein and mutant huntingtin, which were associated with Huntington’s and Parkinson’s disease. SMER 28, which seemed to act either independently or downstream of the rapamycin target, was found to attenuate the mutant huntingtin-fragment toxicity in Huntington’s disease cells .
In vivo: Previous study confirmed that the reduction of EGFP-HDQ74 aggregation occured through autophagy using autophagy-competent mouse embryonic fibroblasts (MEFs) (Atg5+/+). EGFP-HDQ74 aggregation was increased significantly in untreated Atg5-/- (autophagy-deficient) cells when compared with untreated Atg5+/+ cells. SMER 28 reduced EGFP-HDQ74 aggregation in Atg5+/+ cells significantly, but not in Atg5-/- cells). Therefore, SMER 28 could only reduce mutant huntingtin aggregation in autophagy-competent cells .
Clinical trial: N/A
 Sarkar S,Perlstein EO,Imarisio S,Pineau S,Cordenier A,Maglathlin RL,Webster JA,Lewis TA,O'Kane CJ,Schreiber SL,Rubinsztein DC. Small molecules enhance autophagy and reduce toxicity in Huntington's disease models. Nat Chem Biol.2007 Jun;3(6):331-8.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||insoluble in EtOH; insoluble in H2O; ≥101.4 mg/mL in DMSO|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|