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Smart-Seq2 Single Cell Kit

Catalog No.
K1158
High Sensitivity Single Cell Whole Transcriptome Amplification (WTA) Kit
Grouped product items
SizePriceStock Qty
12 rxns
$300.00
In stock
24 rxns
$520.00
In stock
96 rxns
$1,768.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Description

Smart-Seq2 Single Cell Kit is a highly sensitive cDNA preparation kit that obtains high-quality full-length cDNA from 1-1,000 cells or 10 pg-10 ng total RNA for single cells or trace samples RNA-seq analysis. Smart-Seq2 Single Cell Kit uses Oligo dT30VN primer as the reverse transcription primer to synthesize cDNA, and relies on the template-switching activity of Reverse Transcriptase to add adapter sequence to the 3'-end reverse transcribed full length cDNA. Through subsequent PCR amplification, to obtain full-length cDNA, effectively avoiding the 3' bias and rRNA contamination. The obtained full-length cDNA product can be used for transcriptome sequencing and gene expression analysis.

Smart-Seq2 Single Cell Kit is suitable for the detection of trace samples such as single cells, so that samples with low abundance and low concentration can also be analyzed by RNA-seq sequencing. In general, one reaction can produce 2-20 ng of cDNA amplification products.

Figure 1. Schematic of the technology in the Smart-Seq2 Single Cell Kit

Quality Control

Quality Control & MSDS

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Components and Storage

Components 12 rxns 24 rxns 96 rxns
Box 1 Control Total RNA* (1 μg/μL) 5 μL 5 μL 5 μL
TS Oligo Primer (100 μM) 2.4 μL 4.8 μL 19.2 μL
Box 2 10X Lysis Buffer 230 μL 460 μL 920 μL x 2
Oligo dT30VN Primer (10 μM) 24 μL 48 μL 192 μL
dNTP Mixture (10 mM) 24 μL 48 μL 192 μL
5X First-Strand Buffer 48 μL 96 μL 384 μL
Reverse Transcriptase (200 U/μL) 12 μL 24 μL 96 μL
RNase Inhibitor, Murine (40 U/μL) 30 μL 60 μL 240 μL
Betaine (5 M) 48 μL 96 μL 384 μL
2X HyPerFUsion® High-Fidelity Master Mix 300 μL 600 μL 1 mL x 3
IS PCR Primer (10 μM) 6 μL 12 μL 48 μL
Elution Buffer 1 mL 1 mL 1 mL x 2
Nuclease-free Water 1 mL 1 mL 1 mL x 2

Box 1: Store at -80°C.

Box 2: Store at -20°C.

*Note: The Control Total RNA is from Hela cell.

Features

Low starting amount of template:Prepare high-quality full-length cDNA with 1-1,000 cells or 10 pg-10 ng of total RNA template.

High sensitivity of amplification: Combined with Smart-Seq2 and LNA technology, more genes can be detected.

Easy to operate: Single-tube protocol from cell lysis to cDNA, reducing operation steps and operation time.

High success rate of operation: Fewer RNA sample processing steps to avoid the risk of sample loss and increase the success rate of the operation.

Storage

Box 1: Store at -80°C. Box 2: Store at -20°C.