Nanchangmycin

Cell lines

U2OS cells, Vero cells

Preparation method

The solubility of this compound in DMSO is > 10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

2 μM

Applications

Nanchangmycin was modestly toxic but very efficacious in inhibiting viral infection. Nanchangmycin blocked ZIKV infection with low toxicity in the sub-micromolar range. Nanchangmycin potently blocked ZIKV infection across cell types. The IC50 values for infection were between 0.1 and 0.4 μM. Nanchangmycin inhibited DENV across cell types. In Vero cells, treatment with nanchangmycin reduced viral titers by 4-log. Nanchangmycin blocked an early step in the viral life cycle. In U2OS cells, treatment with nanchangmycin (2 μM) blocked infection by an additional flavivirus (WNV, Kunjin) or alphaviruses (CHIKV, 181/25; SINV, HRsp). In HBMECs, nanchangmycin effectively blocked ZIKV infection in HUVECs and UtMECs. Nanchangmycin potently inhibited ZIKV infection in primary placental fibroblast cells.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Rausch K, Hackett B A, Weinbren N L, et al. Screening bioactives reveals nanchangmycin as a broad spectrum antiviral active against Zika virus[J]. Cell reports, 2017, 18(3): 804-815.