Verapamil HCl
Verapamil is an L-type calcium channel blocker of thephenylalkylamine class.
- 1. René Rasche, Björn Udo Klink, et al. "Structure and mechanism of the RalGAP tumor suppressor complex." bioRxiv. November 25, 2024.
- 2. Kai Thoris, Miguel Correa Marrero, et al. "Uncoupling FRUITFULL's functions through modification of a protein motif identified by co-ortholog analysis." Nucleic Acids Res. 2024 Oct 30:gkae963. PMID: 39475190
- 3. Xijun Zhu, Woong Sub Byun, et al. "Activating p53Y220C with a Mutant-Specific Small Molecule." bioRxiv. October 28, 2024.
- 4. Zhewen Dong, Xiaofei She, et al. "The E3 ligase NEDD4L prevents colorectal cancer liver metastasis via degradation of PRMT5 to inhibit the AKT/mTOR signaling pathway." bioRxiv. October 24, 2024.
| Storage | Store at -20°C |
| M.Wt | 491.06 |
| Cas No. | 152-11-4 |
| Formula | C27H39ClN2O4 |
| Solubility | ≥14.45 mg/mL in DMSO; ≥6.41 mg/mL in H2O with ultrasonic; ≥8.95 mg/mL in EtOH with ultrasonic |
| Chemical Name | 2-(3,4-dimethoxyphenyl)-5-[2-(3,4-dimethoxyphenyl)ethyl-methylamino]-2-propan-2-ylpentanenitrile;hydrochloride |
| SDF | Download SDF |
| Canonical SMILES | CC(C)C(CCCN(C)CCC1=CC(=C(C=C1)OC)OC)(C#N)C2=CC(=C(C=C2)OC)OC.Cl |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment [1]: | |
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Cell lines |
myeloma cell lines (JK-6L, RPMI8226, and ARH-77 cell lines) |
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Preparation method |
The solubility of this compound in DMSO is >14.5mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
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Reacting condition |
70 μM; 16 h |
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Applications |
In myeloma cell lines, the combination of bortezomib (10 nM) and verapamil (70 μM) markedly reduced the viability of the JK-6L, RPMI8226, and ARH-77 cell lines. JK-6L cells were more sensitive toward bortezomib and verapamil treatment. Combination of bortezomib and verapamil might induce predominantly apoptotic cell death and activation of caspase 3/7. |
| Animal experiment [2]: | |
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Animal models |
The collagen-induced arthritis (CIA) mice model |
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Dosage form |
20 mg/kg; intraperitoneally every day starting on day 21 |
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Application |
In CIA mice model, verapamil remarkably attenuated development of arthritis and alleviated inflammation. Verapamil also significantly reduced mRNA levels of inflammation-associated molecules, including IL-1β, IL-6, NOS-2, and COX-2. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1]. Meister S1, Frey B, Lang VR, et al. Calcium channel blocker verapamil enhances endoplasmic reticulum stress and cell death induced by proteasome inhibition in myeloma cells. Neoplasia. 2010 Jul;12(7):550-61. [2]. Wang W1, Li Z2, Meng Q3, et al. Chronic Calcium Channel Inhibitor Verapamil Antagonizes TNF-α-Mediated Inflammatory Reaction and Protects Against Inflammatory Arthritis in Mice. Inflammation. 2016 Oct;39(5):1624-34. |
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Quality Control & MSDS
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Chemical structure
Related Biological Data
Related Biological Data
