Scriptaid
Scriptaid, identified by a high-throughput transcriptional screening, is a novel histone deacetylase (HDAC) with specific potency towards class I HDACs (50% inhibition concentration IC50 values of 0.6 μM for HDAC1 and HDAC3 and 1 μM for HDAC8). Scriptaid shares a similar chemical structure with several others hydroxamic acid-containing HDAC inhibitors (such as TSA and nullscript), which consists of a hydroxamic acid group, an aliphatic chain and an aromatic cap at the other end. Scriptaid has the potential to be used for the treatment of glioblastoma multiforme (GBM), one of the most challenging solid cancers to treat, for its ability to induce apoptosis in glioblastoma cells.
Reference
Sharma V, Koul N, Joseph C, Dixit D, Ghosh S, Sen E. HDAC inhibitor, scriptaid, induces glioma cell apoptosis through JNK activation and inhibits telomerase activity. J Cell Mol Med. 2010;14(8):2151-2161.
Hu E, Dul E, Sung CM, Chen Z, Kirkpatrick R, Zhang GF, Johanson K, Liu R, Lago A, Hofmann G, Macarron R, de los Frailes M, Perez P, Krawiec J, Winkler J, Jaye M. Identification of novel isoform-selective inhibitors within class I histone deacetylases. J Pharmacol Exp Ther. 2003;307(2):720-728.
Su GH, Sohn TA, Ryu B, Kern SE. A novel histone deacetylase inhibitor identified by high-throughput transcriptional screening of a compound library. Cancer Res. 2000; 60(12):3137-3142.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 326.35 |
| Cas No. | 287383-59-9 |
| Formula | C18H18N2O4 |
| Solubility | insoluble in H2O; ≥13.1 mg/mL in DMSO; ≥3.17 mg/mL in EtOH with gentle warming and ultrasonic |
| Chemical Name | 6-(1,3-dioxobenzo[de]isoquinolin-2-yl)-N-hydroxyhexanamide |
| SDF | Download SDF |
| Canonical SMILES | C1=CC2=C3C(=C1)C(=O)N(C(=O)C3=CC=C2)CCCCCC(=O)NO |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment [1]: | |
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Cell lines |
MDA-MB-231 cell lines |
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Preparation method |
The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while.Stock solution can be stored below -20°C for several months. |
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Reaction Conditions |
48 h; 1.0 mg/mL |
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Applications |
Based on MTT assay a concentration of 1mg/ml of Scriptaid was chosen for the experiments in MDA-MB-231. MDA-MB-231 cells were treated with 0.1, 0.5, and 1.0 mg/mL of Scriptaid for 48 h. A dose-dependent increase in α-ER mRNA was detectable with concentrations as low as 0.1mg/ml in MDA-MB-231. Maximal α-ER mRNA was detected at 1.0mg/ml. |
| Animal experiment [2]: | |
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Animal models |
B6D2F1 male and female mice; SCNT embryos |
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Dosage form |
250 nM; immersion |
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Applications |
Treating SCNT embryos with HDAC inhibitor, scriptaid, all the important inbred mouse strains can be cloned, such as C57BL/6, C3H/He, DBA/2, and 129/Sv. Normal development, reproductive ability, and genotype in cloned inbred mice produced by scriptaid treatment. Scriptaid has also lower toxicity for embryo development that treatment of ICSI-fertilized embryos with 250 nM scriptaid, for up to 48 h, did not inhibit in vitro or in vivo development. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1] Giacinti L, Giacinti C, Gabellini C, et al. Scriptaid effects on breast cancer cell lines[J]. Journal of cellular physiology, 2012, 227(10): 3426-3433. [2] Van Thuan N, Bui H T, Kim J H, et al. The histone deacetylase inhibitor scriptaid enhances nascent mRNA production and rescues full-term development in cloned inbred mice[J]. Reproduction, 2009, 138(2): 309-317. |
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| Description | Scriptaid is an inhibitor of HDAC. | |||||
| Targets | HDAC | |||||
| IC50 | ||||||
Quality Control & MSDS
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Chemical structure
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Related Biological Data
