SB-3CT

mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail

Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.

Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody

Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay

SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.

Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
SB-3CT is a potent and selective inhibitor of gelatinases with Ki values of 13.9 and 600 nM for MMP-2 and MMP-9, respectively.
Gelatinases A (MMP-2) and B (MMP-9) belong to matrix metalloproteinases family and are involved in tumor metastasis and angiogenesis by hydrolyzing extracellular matrix [1].
SB-3CT is a potent and selective gelatinases inhibitor. SB-3CT was a novel mechanism-based inhibitor that directly bound to the catalytic zinc ion of MMP-2 [1]. SB-3CT inhibited purified mouse MMP-9 with Ki value of 120 nM [2].
In mice bearing T-cell lymphoma L-CI.5s cells, SB-3CT reduced the number of liver metastases in a dose dependent way and reduced the number of tumor colonies by >70% at 50 mg/kg/d. SB-3CT (50 mg/kg/d) also significantly reduced colony size and the number of PCNA positive tumor cells in the livers. These results suggested that SB-3CT effectively inhibited tumor cell extravasation and exhibited an antiproliferative effect. SB-3CT completely inhibited MMP-mediated gelatinolytic activity through the inhibition of both MMP-2 and MMP-9. Also, SB-3CT increased survival [2]. In a transient focal cerebral ischemia mice model, SB-3CT inhibited laminin cleavage mediated by MMP-9 and then rescued neurons from apoptosis [3].
References:
[1]. Kleifeld O, Kotra LP, Gervasi DC, et al. X-ray absorption studies of human matrix metalloproteinase-2 (MMP-2) bound to a highly selective mechanism-based inhibitor. comparison with the latent and active forms of the enzyme. J Biol Chem, 2001, 276(20): 17125-17131.
[2]. Krüger A, Arlt MJ, Gerg M, et al. Antimetastatic activity of a novel mechanism-based gelatinase inhibitor. Cancer Res, 2005, 65(9): 3523-3526.
[3]. Gu Z, Cui J, Brown S, et al. A highly specific inhibitor of matrix metalloproteinase-9 rescues laminin from proteolysis and neurons from apoptosis in transient focal cerebral ischemia. J Neurosci, 2005, 25(27): 6401-6408.
Physical Appearance | A solid |
Storage | Desiccate at -20°C |
M.Wt | 306.40 |
Cas No. | 292605-14-2 |
Formula | C15H14O3S2 |
Solubility | ≥30.6 mg/mL in DMSO; insoluble in H2O; ≥2.43 mg/mL in EtOH |
Chemical Name | 2-(((4-phenoxyphenyl)sulfonyl)methyl)thiirane |
SDF | Download SDF |
Canonical SMILES | O=S(CC1CS1)(C2=CC=C(OC3=CC=CC=C3)C=C2)=O |
Shipping Condition | Ship with blue ice, or upon other requests. |
General tips | For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Chemical structure
