Saccharin 1-methylimidazole (SMI)
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Saccharin 1-methylimidazole (SMI) is a DNA and RNA synthesis activator.
Saccharin 1-methylimidazole (SMI) is the saccharin salt of 1-methylimidazole. 1-Methylimidazole or N-methylimidazole is identified as an aromatic heterocyclic organic agent with the formula CH3C3H3N2. 1-Methylimidazole is a colourless liquid that is used as a base, a specialty solvent, and as a precursor to some ionic liquids. 1-Methylimidazole is a fundamental nitrogen heterocycle and 1-methylimidazole mimics for a variety of nucleoside bases as well as histidine and histamine.
In vitro: Saccharin 1-methylimidazole was found to be able to perform very well for DNA and RNA synthesis. In addition, by using 3 minute, 6 minute, and 12 minute coupling times, saccharin 1-methylimidazole was evaluated in RNA synthesis using TBDMS phosphoramidites, and the results found that a 6 minute coupling time for saccharin 1-methylimidazole showed ETT at a 6 minute coupling time. In additio, it was found that longer coupling times for Saccharin 1-methylimidazole could result in even further improvements in RNA coupling efficiencies .
In vivo: So far, there is no animal in vivo data reported for saccharin 1-methylimidazole.
Clinical trial: Up to now, saccharin 1-methylimidazole is not used clinically.
 Russell MA, Laws AP, Atherton JH, Page MI. The mechanism of the phosphoramidite synthesis of polynucleotides. Org Biomol Chem. 2008 Sep 21;6(18):3270-5.
|Storage||Store at -20°C|
|Solubility||Soluble in DMSO|
|Chemical Name||1-methyl-1H-imidazol-1-ium benzo[d]isothiazol-3-olate 1,1-dioxide|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|