In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Ruthenium red is a strong inhibitor of Ca2+ transport in mitochondrial, in erythrocyte membrane and in sarcoplasmic reticulum (SR) of rabbit skeletal muscle, with the Km of 4.5 μM and 2.0 mM for the two Ca2+-binding sites .
By kinetic experiments, there are two high-affinity Ca2+-binding sites face the cytoplasmic portion of the SR membranes, which are located in helical segments clustered in the transmembrane domain of Ca2+-ATPase, forming a Ca2+ channel.
SR vesicles were incubated with 10 μM-45Ca2+ and various ruthenium red concentrations for 15 min before being filtered for 45Ca counting. The titration curve of Ca2+ binding showed two well-differentiated saturating effects with the Km of 4.5 μM and 2.0 mM for the two Ca2+-binding sites. The ability of SR vesicles to bind Ca2+ was decreased by micromolar ruthenium red concentrations .
In the investigation of the ability of inhibiting caspsaicin-induced plasma extravasation in the rat trachea, ruthenium red can reduce the amount of plasma extravasation in dose-dependently, with a dose of 5 μmol/kg of completely inhibiting .
 Corbalan-Garcia S, Teruel J A, Gomez-Fernandez J C. Characterization of Ruthenium Red-binding sites of the Ca2+-ATPase from sarcoplasmic reticulum and their interaction with Ca2+-binding sites [J]. Biochem J. 1992, 287(3): 767-774.
 Brokaw J J, White G W. Characterization of Ruthenium Red as an Inhibitor of Neurogenic Inflammation in the Rat Trachea [J]. Gen Pharmac. 1995, 26(2): 327-331.
|Physical Appearance||A solid|
|Storage||Store at RT|
|Solubility||insoluble in DMSO; insoluble in EtOH; insoluble in H2O|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|