Recombinant Rhesus Macaque Serum Amyloid A1
Serum Amyloid A protein-1 belongs to the SAA family of apolipoproteins that circulates in association with high-density lipoproteins (HDL). It is a multifunctional protein produced by hepatocytes in response to pro-inflammatory cytokines. SAA1 is a ligand for CD36/SR-B3, SR-B1, FPRL1, TLR2, and RAGE on monocytes/macrophages, inducing chemotaxis and generation of cytokines and tissue factor. It also can bind the surface of invading gram-negative bacteria, acting as an opsonin to aid clearance by macrophages. Additionally, SAA1 binds platelets, probably by engaging fibrinogen on the platelet surface.
Reference
1. Stevens G, Ramsay M, Kluve-Beckerman B, et al. 1993. Genomics, 15: 242-3
2. Kluve-Beckerman BandSong M. 1995. Gene, 159: 289-90
3. Kluve-Beckerman B, Malle E, Vitt H, et al. 1991. Biochem Biophys Res Commun, 181: 1097-102
4. Milan E, Lazzari C, Anand S, et al. 2012. J Proteomics, 76 Spec No.: 91-10
1.
| Gene ID | 694827 |
| Accession # | F6V9N7 |
| Alternate Names | Amyloid fibril protein AA |
| Source | Escherichia coli. |
| M.Wt | Approximately 11.7 kDa, a single non-glycosylated polypeptide chain containing 104 amino acids. |
| AA Sequence | RSWFSFLGEA YDGARDMWRA YSDMKEANYK NSDKYFHARG NYDAAQRGPG GVWAAEVISD ARENIQKLLG RGAEDTLADQ AANEWGRSGK DPNHFRPAGL PEKY |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 97 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rRhSAA1 as determined by LAL method.