Recombinant Rhesus Macaque Interleukin-16
Interleukin-16 (IL-16) is also called lymphocyte chemoattractant factor (LCF) and it is mostly secreted by lymphocytes, epithelial cells, eosinophils, and CD8+ T cells. It has many functions including induction of the IL-2Rα on T cells, suppression of human immunodeficiency virus (HIV) replication, inhibition of T-cell antigen receptor/CD3 mediated T-cell stimulation in mixed lymphocyte reactions and so on. It signals through CD4 receptor. Furthermore, recombinant rhesus macaque interleukin-16 contains 121 amino acid residues and it shares 85 % ~ 95 % a.a. sequence identity with human and murine IL-16.
Reference
1. Baier M, Bannert N, Werner A, et al. 1997. Proc Natl Acad Sci U S A, 94: 5273-7
2. Zhang Y, Center DM, Wu DM, et al. 1998. J Biol Chem, 273: 1144-9
3. Cruikshank WW, Kornfeld H, Center DM. 2000. J Leukoc Biol, 67: 757-66
4. Cruikshank WW, Center DM, Nisar N, et al. 1994. Proc Natl Acad Sci U S A, 91: 5109-13
5. Mathy NL, Scheuer W, Lanzendorfer M, et al. 2000. Immunology, 100: 63-9.
| Gene ID | 574100 |
| Accession # | O62675 |
| Alternate Names | Interleukin-16, Lymphocyte Chemoattractant Factor, LCF |
| Source | Escherichia coli. |
| M.Wt | Approximately 12.5 kDa, a single non-glycosylated polypeptide chain containing 121 amino acids. |
| AA Sequence | SAASASAASD VSVESSAEAT VYTVTLEKMS AGLGFSLEGG KGSLHGDKPL TINRIFKGAA SEQSETIQPG DEILQLAGTA MQGLTRFEAW NIIKALPDGP VTIVIRRKSL QPKETTAAAD S |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral T lymphocytes is in a concentration range of 1.0-100 ng/ml. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 98 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rRhIL-16 as determined by LAL method.