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Propidium iodide

Catalog No.
B7758
A red-fluorescent DNA stain
Grouped product items
SizePriceStock Qty
10mg
$50.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

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Background

Propidium iodide (CAS 25535-16-4), commonly abbreviated as PI, is a fluorescent nucleic acid intercalating dye widely employed in biomedical research. Structurally analogous to ethidium bromide, PI intercalates into double-stranded DNA molecules without sequence specificity, binding approximately one dye per 4–5 base pairs. Due to membrane impermeability, PI selectively enters cells only when plasma membrane integrity is compromised, as occurs during cell death or late apoptosis. Upon DNA binding, its fluorescence notably increases, facilitating detection via fluorescence microscopy, spectrometry, or flow cytometry. PI is routinely utilized for distinguishing necrotic or late apoptotic cells in viability assays, apoptosis studies (often paired with Annexin V), and cell cycle analyses.

Product Citation

Chemical Properties

Physical AppearanceA crystalline solid
StorageStore at -20°C
M.Wt668.39
Cas No.25535-16-4
FormulaC27H34I2N4
Solubilityinsoluble in H2O; insoluble in EtOH; ≥9.84 mg/mL in DMSO
Chemical Name3,8-diamino-5-(3-(diethyl(methyl)ammonio)propyl)-6-phenylphenanthridin-5-ium iodide
SDFDownload SDF
Canonical SMILESNC1=CC2=[N+](C(C3=CC=CC=C3)=C(C=C4N)C(C=C4)=C2C=C1)CCC[N+](CC)(C)CC.[I-].[I-]
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

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