Phalloidin
Phalloidin, a mushroom-derived toxin, can be used to label cytoskeletal F-actin with fluorochrome (λex = 495 nm, λem = 520 nm). Also, phalloidin can react stoichiometrically with muscle actin, substantially promote actin polymerization, and stabilize polymerized actin. Actin is the major structural protein of the cytoplasm of eukaryotic cells, and a well-balanced equilibrium between different polymerization states of actin is important for various cellular functions such as cell locomotion and cell growth.
References:
1. Chazotte B. Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging. Cold Spring Harb Protoc. 2010 May;2010(5):pdb.prot4947.
2. Wehland J, Osborn M, Weber K. Phalloidin-induced actin polymerization in the cytoplasm of cultured cells interferes with cell locomotion and growth. Proceedings of the National Academy of Sciences of the United States of America, 1977, 74(12): 5613-5617.
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- 2. Mengliu Luo, MD, et al. "Yo-Yo Dieting Delays Male Drosophila melanogaster Aging Through Enhanced Mitochondrial Function, Relative to Sustained High-Calorie Diet Feeding." J Gerontol A Biol Sci Med Sci. 2024 Apr 1;79(4):glae014. PMID: 38198696
- 3. Wang X, Cai Y, et al. "Effect of Biodentine on Odonto/Osteogenic Differentiation of Human Dental Pulp Stem Cells." Bioengineering (Basel) 2022 Dec 21;10(1). PMID: 36671584
| Physical Appearance | A crystalline solid |
| Storage | Store at -20°C |
| M.Wt | 788.87 |
| Cas No. | 17466-45-4 |
| Formula | C35H48N8O11S |
| Solubility | Soluble to 1 mg/ml in sterile water |
| Chemical Name | A name could not be generated for this structure. |
| SDF | Download SDF |
| Canonical SMILES | O[C@@H]1C[C@H](C(N[C@@H](C)C(N[C@@H](C(N[C@@H](C(N[C@@H]2C)=O)C[C@](CO)(C)O)=O)CC3=C(NC4=CC=CC=C34)SC[C@H]5NC([C@H]([C@H](C)O)NC2=O)=O)=O)=O)N(C5=O)C1 |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment:[2] | |
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Cell lines |
Mouse 3T3 and rat kangaroo PtK2 cells |
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Reaction Conditions |
0.2 or 1 mM phalloidin in 0.14 M KCI (Me2SO concentration 0.4 or 2%), for 3 h incubation |
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Applications |
When phalloidin was injected into cells, it recruited the less highly polymerized forms of actin and/or G actin, to form "islands" of aggregated actin in a focal plane above the stress fibers. In addition, microinjection of phalloidin interfered cell locomotion and cell growth in a concentration-dependent manner. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Chazotte B. Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging. Cold Spring Harb Protoc. 2010 May;2010(5):pdb.prot4947. 2. Wehland J, Osborn M, Weber K. Phalloidin-induced actin polymerization in the cytoplasm of cultured cells interferes with cell locomotion and growth. Proceedings of the National Academy of Sciences of the United States of America, 1977, 74(12): 5613-5617. |
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Quality Control & MSDS
- View current batch:
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Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
