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Phalloidin

Catalog No.
B7678
A high affinity probe for F-actin, which also promotes actin polymerization
Grouped product items
SizePriceStock Qty
1mg
$301.00
Ship with 5-10 days
For scientific research use only and should not be used for diagnostic or medical purposes.

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Email: [email protected]

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Background

Phalloidin (CAS 17466-45-4) is a cyclic heptapeptide toxin originally isolated from the Amanita phalloides mushroom, which specifically binds filamentous actin (F-actin) with high affinity (Kd approximately 20 nM), without binding to monomeric actin (G-actin). By binding directly to F-actin, phalloidin stabilizes these filaments and prevents their depolymerization, thereby disrupting the dynamic equilibrium essential for normal cytoskeletal processes. Conjugates of phalloidin with fluorophores such as FITC or Cy dyes are widely utilized in microscopy-based applications, enabling selective and species-independent visualization of F-actin organization in fixed cells, permeabilized samples, tissue sections, and cell-free assays.

Product Citation

Chemical Properties

Physical AppearanceA crystalline solid
StorageStore at -20°C
M.Wt788.87
Cas No.17466-45-4
FormulaC35H48N8O11S
SolubilitySoluble to 1 mg/ml in sterile water
Chemical NameA name could not be generated for this structure.
SDFDownload SDF
Canonical SMILESO[C@@H]1C[C@H](C(N[C@@H](C)C(N[C@@H](C(N[C@@H](C(N[C@@H]2C)=O)C[C@](CO)(C)O)=O)CC3=C(NC4=CC=CC=C34)SC[C@H]5NC([C@H]([C@H](C)O)NC2=O)=O)=O)=O)N(C5=O)C1
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment:[2]

Cell lines

Mouse 3T3 and rat kangaroo PtK2 cells

Reaction Conditions

0.2 or 1 mM phalloidin in 0.14 M KCI (Me2SO concentration 0.4 or 2%), for 3 h incubation

Applications

When phalloidin was injected into cells, it recruited the less highly polymerized forms of actin and/or G actin, to form "islands" of aggregated actin in a focal plane above the stress fibers. In addition, microinjection of phalloidin interfered cell locomotion and cell growth in a concentration-dependent manner.

Note

The technical data provided above is for reference only.

References:

1. Chazotte B. Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging. Cold Spring Harb Protoc. 2010 May;2010(5):pdb.prot4947.

2. Wehland J, Osborn M, Weber K. Phalloidin-induced actin polymerization in the cytoplasm of cultured cells interferes with cell locomotion and growth. Proceedings of the National Academy of Sciences of the United States of America, 1977, 74(12): 5613-5617.

Quality Control

Quality Control & MSDS

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Chemical structure

Phalloidin