In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Oxy-16 is an antagonist of hedgehog activity.
Naturally occurring oxysterols that are products of cholesterol oxidation can stimulate the hedgehog (Hh) signaling pathway related to cardiovascular disease and bone formation. Activation of Hh signaling modulates inflammatory responses to additional atherogenic factors including lesion-producing macrophages and enable osteoblast differentiation, which is dependent on the target cell.
In vitro: Oxysterols have been shown to be associated with immunosuppression, apoptosis, atherosclerosis, inflammation, and cholesterol turnover. Other studies aslo indicated that oxysterols were Hedgehog (Hh) signaling pathway activators and had potent osteoinductive properties. Oxy-16, an oxysterol, was identified as a cell permeable oxygenated derivative of cholesterol that might be the end product or intermediate of the cholesterol excretion pathways. Oxy-16 is currently used as a transport form for cholesterol across the blood brain barrier and membranes. The osteogenic differentiation caused by Oxy-16 was cinsidered to be mediated via a Wnt signaling-related, Dkk-1-inhibitable mechanism .
In vivo: Up to now, there is no animal in vivo data reported.
Clinical trial: So far, no clinical study has been conducted.
 Amantea, C. M.,Kim, W.K.,Meliton, V., et al. Oxysterol-induced osteogenic differentiation of marrow stromal cells is regulated by Dkk-1 inhibitable and PI3-kinase mediated signaling. Journal of Cellular Biochemistry 105(2), 424-436 (2008).
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Solubility||≤10mg/ml in ethanol;1.5mg/ml in DMSO;1.5mg/ml in dimethyl formamide|
|Canonical SMILES||O[[email protected]](C1)CC[[email protected]@]2(C)C1=CC[[email protected]]3([H])C2CC[[email protected]@]4(C)[[email protected]@]3([H])CC[[email protected]]4([H])[[email protected]@](C)(O)[[email protected]](O)CCC(C)C|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|