In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Target: ATP synthase
GI50: 270 nM
Oligomycin complex is a mixture of oligomycins A, B, and C. Oligomycin A is a selective inhibitor of ATP synthase, which can inhibit oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria, and induces apoptosis in a variety of cell types. Oligomycin B is a nonselective inhibitor of ATP synthases, which can reduce the rate of ATP depletion in myocardial ischemia.
In Vitro: In A549, H1299, H1975, H520, 786-0, H838, and U87mG cancer cell lines, 100 ng/ml Oligomycin could completely inhibit OXPHOS phosphorylation activity and stimulate various levels of glycolysis gains . Besides, 5 μg/ml Oligomycin could inhibit the F0 part of H+-ATP-synthase, and strongly suppress cytochrome c release and apoptosis in Hela cells induced by TNF . Furthermore, Oligomycin could inhibit mitochondrial respiration, and sensitize melanoma cells to cisplatin treatment to inhibit cell invasion .
In Vivo: no data available.
Clinical trial: no data available.
 Hao W, Chang C B, Tsao C, et al. Oligomycin-induced Bioenergetic Adaptation in Cancer Cells with Heterogeneous Bioenergetic Organization[J]. Journal of Biological Chemistry, 2010, 285(17): 12647-12654.
 Shchepina L A, Pletjushkina O Y, Avetisyan A V, et al. Oligomycin, inhibitor of the F0 part of H+-ATP-synthase, suppresses the TNF-induced apoptosis[J]. Oncogene, 2002, 21(53): 8149.
 Roesch A, Vultur A, Bogeski I, et al. Overcoming Intrinsic Multidrug Resistance in Melanoma by Blocking the Mitochondrial Respiratory Chain of Slow-Cycling JARID1Bhigh Cells[J]. Cancer Cell, 2013, 23(6): 811-825.
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|M.Wt||791.1(Oligomycin A) 805.1(Oligomycin B) 775.1(Oligomycin C)|
|Formula||C45H74O11(Oligomycin A) C45H72O12(Oligomycin B) C45H74O10(Oligomycin C)|
|Solubility||≤30mg/ml in ethanol;20mg/ml in DMSO;30mg/ml in dimethyl formamide|
|Canonical SMILES||O=C([[email protected]]([[email protected]@H]([[email protected]](C([[email protected]](C)([[email protected]@H]([[email protected]](C/C=C/C=C/[[email protected]]([H])(CC[[email protected]@]1([[email protected]@H]([[email protected]]([H])(O2)[[email protected]]([[email protected]@]3(O1)C(C[[email protected]]([[email protected]]([H])(O3)C[[email protected]](C)O)C)=O)C)C)[H])CC)C)O)O)=O)C)O)C)[[email protected]@H]([[email protected]]([[email protected]@H](/C=C/C2=O)C)O)C.O=C([[email protected]]([[email protected]@H]([[email protected]](C([[email protected]](C)([[email protected]@H]([[email protected]|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|