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O-propargyl-puromycin (OPP)

Catalog No.
A8778
Used for the detection of protein synthesis
Grouped product items
SizePriceStock Qty
500ug
$252.00
Ship with 10-15 days
1mg
$410.00
Ship with 10-15 days

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

An alkyne derivative of puromycin used to supervise newly protein synthesis, named O-propargyl-puromycin (OPP), is a potent protein synthesis inhibitor that stopping translation through be incorporated into the C-terminus of translating polypeptide chains.

OPP can conjugate with nascent polypeptide chains thus constituting a covalent form. In addition, OPP can be visualised or captured through copper (I)-catalyzed azide-alkyne cycloaddition [1]. Thus, OPP often regarded as a marker suitable for imaging and identifying nascent proteins in cells and animals. The research has reported that in CHO cells, the OPP-labelling could quantify global protein synthesis. Moreover, at the single-cell level, the OPP-labelling enables correlation of global protein synthesis rates with DNA content [2]. In vivo, the injected OPP mice tissues display specific cohesion with nascent proteins than the uninjected mice tissues [1]. OPP-mediated identification (OPP-ID), is a technique to find how cells regulate the nascent protein expression that can apply in most cell types when cells suffer stimuli and stress [3].

 

References:

[1] Liu J, Xu Y, Stoleru D, et al. Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin.[J]. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(2):413.

[2] Nagelreiter F, Coats M, Klanert G, et al. OPP labelling enables total protein synthesis quantification in CHO production cell lines at the single‐cell level [J]. Biotechnology Journal, 2018, 13(4):1700492.

[3] Forester C M, Zhao Q, Phillips N J, et al. Revealing nascent proteomics in signaling pathways and cell differentiation.[J]. Proceedings of the National Academy of Sciences of the United States of America, 2018, 115(10):2353-2358.

Chemical Properties

StorageStore at -20°C
M.Wt495.53
Cas No.1416561-90-4
FormulaC24H29N7O5
SolubilitySoluble in DMSO
Chemical Name(2S)-2-amino-N-((2S,5R)-5-(6-(dimethylamino)-9H-purin-9-yl)-4-hydroxy-2-(hydroxymethyl)tetrahydrofuran-3-yl)-3-(4-(prop-2-yn-1-yloxy)phenyl)propanamide
SDFDownload SDF
Canonical SMILESOC[[email protected]]1O[[email protected]@H](N2C3=NC=NC(N(C)C)=C3N=C2)C(O)C1NC([[email protected]](CC4=CC=C(OCC#C)C=C4)N)=O
Shipping ConditionEvaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

Quality Control

Quality Control & MSDS

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Chemical structure

O-propargyl-puromycin