In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
An alkyne derivative of puromycin used to supervise newly protein synthesis, named O-propargyl-puromycin (OPP), is a potent protein synthesis inhibitor that stopping translation through be incorporated into the C-terminus of translating polypeptide chains.
OPP can conjugate with nascent polypeptide chains thus constituting a covalent form. In addition, OPP can be visualised or captured through copper (I)-catalyzed azide-alkyne cycloaddition . Thus, OPP often regarded as a marker suitable for imaging and identifying nascent proteins in cells and animals. The research has reported that in CHO cells, the OPP-labelling could quantify global protein synthesis. Moreover, at the single-cell level, the OPP-labelling enables correlation of global protein synthesis rates with DNA content . In vivo, the injected OPP mice tissues display specific cohesion with nascent proteins than the uninjected mice tissues . OPP-mediated identification (OPP-ID), is a technique to find how cells regulate the nascent protein expression that can apply in most cell types when cells suffer stimuli and stress .
 Liu J, Xu Y, Stoleru D, et al. Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin.[J]. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(2):413.
 Nagelreiter F, Coats M, Klanert G, et al. OPP labelling enables total protein synthesis quantification in CHO production cell lines at the single‐cell level [J]. Biotechnology Journal, 2018, 13(4):1700492.
 Forester C M, Zhao Q, Phillips N J, et al. Revealing nascent proteomics in signaling pathways and cell differentiation.[J]. Proceedings of the National Academy of Sciences of the United States of America, 2018, 115(10):2353-2358.
|Storage||Store at -20°C|
|Solubility||Soluble in DMSO|
|Canonical SMILES||OC[[email protected]]1O[[email protected]@H](N2C3=NC=NC(N(C)C)=C3N=C2)C(O)C1NC([[email protected]](CC4=CC=C(OCC#C)C=C4)N)=O|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|