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Catalog No.
Amine-reactive biotinylation reagent, long chain
Grouped product items
SizePriceStock Qty
Ship with 10-15 days
Ship with 10-15 days

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NHS-12-Biotin is a derivative of D-biotin, a amine-reactive biotinylation agent, that contains a spacer arm off the valeric acid side chain of D-biotin with an NHS ester group at its end. The NHS ester group at the end of NHS-12-Biotin covalently binds to amine groups in proteins and other molecules forming a stable amide linkage and releasing the NHS group. NHS-12-Biotin has a greater length between a covalently modified molecule and the bicyclic biotin rings than NHS-LC-Biotin, which provides the avidin or streptavidin probes a greater opportunity to reach the binding sites deeply buried inside the surface plane of proteins. NHS-12-Biotin is insoluble in aqueous environments requiring the dissolution of organic solvents prior to the addition to a buffered reaction.

Chemical Properties

StorageStore at -20°C
Solubility≥20.65 mg/mL in EtOH; ≥23.15 mg/mL in H2O; ≥44.5 mg/mL in DMSO
Chemical Name2,5-dioxopyrrolidin-1-yl 4-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)butanoate
SDFDownload SDF
Canonical SMILESO=C1N[[email protected]@]([H])([[email protected]]([H])(CS2)N1)[[email protected]@H]2CCCCC(NCCCCCC(NCCCC(ON3C(CCC3=O)=O)=O)=O)=O
Shipping ConditionShip with blue ice, or upon other requests.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.

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