In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Neoruscogenin is a bioavailable, potent, and high-affinity agonist of the nuclear receptor RORα . Neoruscogenin is a natural sapogenin extracted from Butcher’s broom (Rusci rhizoma, Ruscus aculeatus, Ruscaceae) .
RAR-related orphan receptor alpha (RORα) is a nuclear receptor that participates in the transcriptional regulation of some genes involved incircadian rhythm. Genetic studies have revealed that RORα plays an important role in the development of the central nervous system and has been associated with genetic lesions in mice .
In vitro: In confluent human microvascular endothelial cells (HMEC-1), pre-treatment with neoruscogenin slightly decreased the permeability at concentrations up to 100 μM (71.8%). The EC50 of neoruscogenin against the nuclear receptor RORα was 110 nM .
In vivo: In mice, oral administration of neoruscogenin (3 mg/kg/d) for seven days up-regulated the expression of several RORα-inducible genes in the liver .
 Helleboid S, Haug C, Lamottke K, et al. The identification of naturally occurring neoruscogenin as a bioavailable, potent, and high-affinity agonist of the nuclear receptor RORα (NR1F1)[J]. Journal of biomolecular screening, 2013: 1087057113497095.
 Barbi M, Willer E A, Rothenhfer M, et al. Spirostanol saponins and esculin from Rusci rhizoma reduce the thrombin-induced hyperpermeability of endothelial cells[J]. Phytochemistry, 2013, 90: 106-113.
 Giguère V. Orphan nuclear receptors: from gene to function 1[J]. Endocrine reviews, 1999, 20(5): 689-725.
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Solubility||≤30mg/ml in ethanol;30mg/ml in DMSO;30mg/ml in dimethyl formamide|
|Canonical SMILES||C=C(CO1)CC[[email protected]@]21O[[email protected]@]3([H])C[[email protected]@]4([H])[[email protected]]5([H])CC=C6C[[email protected]@H](O)C[[email protected]@H](O)[[email protected]]6(C)[[email protected]@]5([H])CC[[email protected]]4(C)[[email protected]@]3([H])[[email protected]@H]2C|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|