Nanobacteria Removal Reagent (500×)

Nanobacteria and their complexes are the common contaminants in cell culture and cannot be removed by conventional 0.22 μm filtration. Nanobacteria are small black swimming dots with a motion characteristic similar to Brownian motion under the microscope. Nanobacteria contamination generally has the following characteristics:

• 1) Although the medium is not turbid, a large number of small black spots around the cells and in the culture medium can be seen under the microscope, and the number of small black spots gradually increases with the extension of culture time. Changing the medium or washing the cells does not improve.
• 2) Commonly used antibiotics such as penicillin, streptomycin, gentamicin, etc., are ineffective.
• 3) The nanobacteria can result in slow cell growth, poor condition, vacuolation, and even changes in cell morphology.

Nanobacteria Removal Reagent (500×) is a reagent that can effectively kill nanobacteria in serum, culture medium and cells, and its main component is antibiotic. This reagent also has a certain killing effect on common bacteria, but it is non-toxic to cells, and this reagent hardly affects the proliferation of the cells.

Figure 1: From left to right, untreated contaminated cells, using competitor A's Nanobacteria removal reagent, and using this product. The results showed that the untreated contaminated cells grew slowly, while the growth rate of the cells was significantly faster and comparable with the use of competitor or this product.