In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50: 250 μM: inhibits α-ketoglutarate-dependent enzyme jumonji domain-containing protein 2 (JMJD2) A.
IC50: 500 μM: blocks α-ketoglutarate-dependent enzyme JMJD2C.
IC50: 24 μM: dampens α-ketoglutarate-dependent enzyme JMJD2E.
IC50: 2.1 μM: suppresses the prolyl hydroxylase domain-containing proteins PHD1.
IC50: 5.6 μM: depresses the prolyl hydroxylase domain-containing proteins PHD2.
N-Oxalylglycine, as known as NOG, is a cell permeable inhibitor of α-ketoglutarate-dependent enzymes which include JMJD2A, JMJD2C, and JMJD2E. NOG also blocks the prolyl hydroxylase domain-containing proteins PHD1 and PHD2. Demethylation of the methylated forms of histone 3 lysine 9 (H3K9) and H3K36 can be catalyzed by JMJD2 subfamily of histone demethylases. Since histone demethylases are related to certain diseases, including cancer, selective inhibitors functions as anticancer agents and potential tools for clarifying the biological functions of JMJDs.
In vitro: NOG was identified as a natural product and presented in plants, including Rheum rhabarbarum (rhubarb) and Spinach oleracea (spinach) leaves. However, there was no NOG observed human embryonic kidney cells (HEK 293T) or Escherchia coli. NOG regulated gene expression via blocking 2-Oxoglutarate-dependent oxygenases .
In vivo: Up to now, in vivo study of NOG is still in the development stage.
. Al-Qahtani, K., Jabeen, B., Sekirnik, R., Riaz, N., Claridge, T., Schofield, C., & McCullagh, J. The broad spectrum 2-oxoglutarate oxygenase inhibitor N-oxalylglycine is present in rhubarb and spinach leaves. Phytochemistry. 2015; 117: 456-461.
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Solubility||≤10mg/ml in ethanol;10mg/ml in DMSO;5mg/ml in dimethyl formamide|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|