T4 Gene 32 Protein(T4gp32)
T4 gene 32 protein (T4 GP32 protein) is a single-stranded DNA (ssDNA)-binding protein required for T4 phage DNA replication and repair. It cooperatively binds to and stabilizes the transiently formed ssDNA region and plays an important role in the DNA replication of T4 bacteriophage. The protein is also widely used to identify single-stranded and double-stranded regions of DNA in electron microscopy studies. Recent reports suggest that T4 gene 32 protein can improve restriction enzyme digestion, increase the yield and efficiency of reverse transcription (RT) during RT-PCR, enhance T4 DNA polymerase activity, and increase the yield and specificity of PCR products.
This product is mainly used to increase the yield and extension of reverse transcription, and to increase the yield and specificity of the target fragment during PCR of soil samples, as well as to stabilize and label ssDNA structure.
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Product name |
T4 Gene 32 Protein |
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Molecular weight |
~33,506 Da |
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Source |
An E. coli strain carrying a plasmid that overexpresses the gene 32 protein of T4 phage |
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Concentration |
10 mg/mL |
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Reaction condition |
37℃ (Optimal reaction temperature) |
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Storage buffer |
20 mM Tris-HCl, 100 mM NaCl, 0.5 mM DTT, 1 mM EDTA, 50% Glycerol (pH 8, 25°C) |
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Storage condition |
-20℃ |
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Storage time |
2 years |
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Conditions of Carriage |
Dry ice |