In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Lasalocid is an ionophore antibiotic.
Lasalocid is produced by Streptomyces lasaliensis and is a mixture of several closely related homologue substances A, B, C, D, E. The sum of the lasalocid homologues B, C, D, E is limited to 10% of the total weight of the active substance, lasalocid.
In vitro: A previous study was conducted to compare effects of the combined use of lasalocid and silybin in chicken hepatoma cells and rat myoblasts cell lines cultures. Results showed that in all performed tests, the lowest lasalocid EC50-values were obtained for chicken hepatocytes. In the rat myoblasts cultures, the lowest lasalocid EC50-values were found. Moreover, a lack of silybin cytotoxic effect was proven for the studied cell lines. An interaction between both substances resulted in a great decrease of lasalocid cytotoxicity. The isobolograms and combination index indicated a significant antagonistic nature of silybin effect in the course of lasalocid cytotoxicity .
In vivo: In a animal study, chickens 8 or 9 days old were given a completely purified crystalline amino acid diet. Results showed that the requirement for sulphur-containing amino acids (SAA) was neither increased nor decreased by lasalocid at 125 mg/kg diet. Moreover, lasalocid could cause a significant increase in gain and gain/feed intake when added to diets that were deficient in SAA, but it led to a marked decrease in performance when added to diets adequate in SAA. In addition, at moderate levels of SAA deficiency, lasalocid at 125 mg/kg could neither increase nor decrease the rate and efficiency of weight gain and there was no evidence of the SAA X lasalocid interaction when lasalocid was given in a typical starter diet of maize and soya .
Clinical trial: So far, no clinical study has been conducted.
 Radko, L. ,Cybulski, W., and Rzeski, W. The protective effect of silybin against lasalocid cytotoxic exposure on chicken and rat cell lines. Biomed.Res.Int. 2013, 1-8 (2013).
 Willis GM, Baker DH. Lasalocid-sulfur amino acid interrelationship in the chick. Poult Sci. 1980 Nov;59(11):2538-43.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Synonyms||Antibiotic X 537A,Ionophore X 537A,X 537A|
|Solubility||Soluble in ethanol;methanol;DMSO;dimethyl formamide|
|Chemical Name||6-[(3R,4S,5S,7R)-7-[(2S,3S,5S)-5-ethyl-5-[(2R,5R,6S)-5-ethyltetrahydro-5-hydroxy-6-methyl-2H-pyran-2-yl]tetrahydro-3-methyl-2-furanyl]-4-hydroxy-3,5-dimethyl-6-oxononyl]-2-hydroxy-3-methyl-benzoic acid|
|Canonical SMILES||OC(C1=C(CC[[email protected]@H](C)[[email protected]](O)[[email protected]](C)C([[email protected]@H]([[email protected]]2([H])[[email protected]@H](C)C[[email protected]]([[email protected]]3([H])O[[email protected]@H](C)[[email protected]@](O)(CC)CC3)(CC)O2)CC)=O)C=CC(C)=C1O)=O|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|