In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Lamin fragment has a peptide sequence of Lys-Ala-Gly-Gln-Val-Val-Thr-Ile-Trp.
The lamins are type V intermediate filaments which can be categorized as either A-type (lamin A, C) or B-type (lamin B1, B2) according to homology in sequence, biochemical properties and cellular localization during the cell cycle. Lamin polypeptides have an almost complete α-helical conformation with multiple α-helical domains separated by non-α-helical linkers that are highly conserved in length and amino acid sequence.
Nuclear lamins are intermediate filament-type proteins that are the major building blocks of the nuclear lamina, a fibrous proteinaceous meshwork underlying the inner nuclear membrane. Lamins can also be localized in the nuclear interior, in a diffuse or spotted patter. Lamins also play roles in DNA replication, chromatin organization, spatial arrangement of nuclear pore complexes, nuclear growth, and anchorage of nuclear envelope proteins.
Figure 1: Lamin fragment
1. The Cell: A Molecular Approach, Cooper & Hausman. 5th Edition. Pg. 357
2. Ayelet Margalit, Sylvia Vlcek, Yozef Gruenbaum, Roland Foisner (2005). Breaking and Making of the Nuclear Envelope. Journal of Cellular Biochemistry 95, 454-465
3. Bruce Alberts, et al. Molecular Biology of the Cell (4th edition). Garland Science 676-677
4. Geoffrey M. Cooper, Robert E. Hausman. The Cell, A Molecular Approach (4th edition). Sinauer Associates 356-360
5. Goldman et al.(2002). "Nuclear lamins: building blocks of nuclear architecture". Genes and Development 16,533-547
6. Joanna M. Bridger, Nicole Foeger, Ian R. Kill, Harald Herrmann (2007). The Nuclear Lamina: both a structural framework and a platform for genome organization. FEBS Journal 274, 1354–1361
7. Nico Stuurman, Susanne Heins, Ueli Aebi (1998). Nuclear Lamins: Their Structure, Assembly and Interactions. Journal of Structural Biology 122, 42-46
8. Tripathi K, Muralikrishna B and Parnaik VK (2009) Differential dynamics and stability of lamin A rod domain mutants IJIB, 5(1), 1-8
9. Yozef Gruenbaum, Katherine L. Wilson, Amnon Harel, Michal Goldberg, Merav Cohen (2000). Nuclear Lamins – Structural Proteins with fundamental functions. Journal of Structural Biology129, 313-323
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||≥100.1 mg/mL in DMSO; insoluble in EtOH; ≥22 mg/mL in H2O|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|