In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
JNJ-1661010, a piperazinyl phenyl urea with its structure distinct from alkylcarbamic acid esters and α–ketoheterocyclic compounds, is a potent and selective inhibitor of fatty acid amide hydrolase (FAAH) that covalently binds to the active site of FAAH through cleavage of the urea functionality generating aniline and a FAAH-poperazinyl carbamate. The inhibition of JNJ-1661010 against FAAH is reversible through the hydrolysis of the carbamate leading to the release of the piperazinyl fragment and the restoration of enzymatic functionality of FAAH. According to results of previous studies, JNJ-1661010 displays its inhibition against recombinant FAAH with values of 50% inhibition concentration IC50 of 10 nM and 12 nM in rat and human respectively.
Karbarz MJ, Luo L, Chang L, Tham CS, Palmer JA, Wilson SJ, Wennerholm ML, Brown SM, Scott BP, Apodaca RL, Keith JM, Wu J, Breitenbucher JG, Chaplan SR, Webb M. Biochemical and biological properties of 4-(3-phenyl-[1,2,4] thiadiazol-5-yl)-piperazine-1-carboxylic acid phenylamide, a mechanism-based inhibitor of fatty acid amide hydrolase. Anesth Analg. 2009;108(1):316-329.
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Solubility||Soluble in DMSO|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|
|Description||JNJ-1661010 is a selective inhibitor of FAAH with IC50 values of 33 nM and 34 nM in human and rat, respectively.|
|IC50||33 nM (human)||34 nM (rat)|