In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IWP-L6 is an inhibitor of Porcupine with IC50 value of 0.5nM .
IWP-L6 is developed as a sub-nanomolar inhibitor.of Porcupine. It targets the Wnt signaling since Porcupine is the enzyme to catalyze the palmitoylation of Wnt proteins. In HEK293 cells, IWP-L6 is found to inhibi the phosphorylation of dishevelled 2 (Dvl2) significantly. IWP-L6 has good stability in human plasma but weaker stability in rat and mouse plasma. In vivo assay shows that IWP-L6 is quite active in zebrafish. It blocks the regeneration of the tailfin effectively. It also shows inhibition of posterior axis formation at low micromolar concentrations. Additionally, in cultured mouse embryonic kidneys, 10nM IWP-L6 can significantly reduce branching morphogenesis while 50nM IWP-L6 completely blocks Wnt signaling .
 Wang X, Moon J, Dodge ME, Pan X, Zhang L, Hanson JM, Tuladhar R, Ma Z, Shi H, Williams NS, Amatruda JF, Carroll TJ, Lum L, Chen C. The development of highly potent inhibitors for porcupine. J Med Chem. 2013 Mar 28;56(6):2700-4.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||≥22.45mg/mL in DMSO|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
|Description||IWP-L6 is a highly potent inhibitor of Porcn with an EC50 value of 0.5 nM.|