HPF
Catalog No.
C3384
HPF is a stable fluorescent reagent for detecting high levels of reactive oxygen species (ROS).
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HPF (CAS 359010-69-8), hydroxyphenyl fluorescein, is a cell-permeable aromatic aminofluorescein derivative exhibiting minimal intrinsic fluorescence. Upon oxidation by highly reactive oxygen species (hROS), such as hydroxyl radicals, peroxynitrite, or those enzymatically generated via peroxidase/H?O? systems, HPF is converted into fluorescein, emitting strong green fluorescence (excitation/emission maxima: 490/515 nm). Notably, HPF does not respond to hypochlorite, nitric oxide, hydrogen peroxide, or superoxide ions, demonstrating specificity in detecting hROS. It is suitable for visualizing intracellular oxidative stress using fluorescence microscopy, microplate readers, high-throughput imaging systems, and flow cytometry.
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| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 424.4 |
| Cas No. | 359010-69-8 |
| Formula | C26H16O6 |
| Synonyms | Hydroxyphenyl fluorescein; 3'-p-(Hydroxyphenyl) fluorescein |
| Solubility | ≤20mg/ml in ethanol; 20mg/ml in DMSO; 20mg/ml in dimethyl formamide |
| Chemical Name | 3'-hydroxy-6'-(4-hydroxyphenoxy)-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one |
| Canonical SMILES | Oc(cc1)ccc1Oc1cc(Oc2c(C3(c4c5cccc4)OC5=O)ccc(O)c2)c3cc1 |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Chemical structure
