HPF
Catalog No.
C3384
HPF is a stable fluorescent reagent for detecting high levels of reactive oxygen species (ROS).
Featured Products
HPF (CAS 359010-69-8), hydroxyphenyl fluorescein, is a cell-permeable aromatic aminofluorescein derivative exhibiting minimal intrinsic fluorescence. Upon oxidation by highly reactive oxygen species (hROS), such as hydroxyl radicals, peroxynitrite, or those enzymatically generated via peroxidase/H?O? systems, HPF is converted into fluorescein, emitting strong green fluorescence (excitation/emission maxima: 490/515 nm). Notably, HPF does not respond to hypochlorite, nitric oxide, hydrogen peroxide, or superoxide ions, demonstrating specificity in detecting hROS. It is suitable for visualizing intracellular oxidative stress using fluorescence microscopy, microplate readers, high-throughput imaging systems, and flow cytometry.
- 1. Hao Dai, Ali Han, et al. "NIR-triggering cobalt single-atom enzyme switches off-to-on for boosting the interactive dynamic effects of multimodal phototherapy." Nat Commun. 2025 Feb 28;16(1):2058. PMID: 40021633
- 2. Chuanlin Feng, Lei Zhang, et al. "Redox imbalance drives magnetic property and function changes in mice." Redox Biol. 2025 Apr:81:103561. PMID: 40020452
- 3. Yumeng Lian, Yi Wang, et al. "Peroxidase-like and oxidase-like nanozyme activities of reusable Mn-Co-S-Se/Ni foam for antibacterial application." Colloids and Surfaces A: Physicochemical and Engineering Aspects. Volume 626, 5 October 2021, 127010.
- 4. CHEN Chao, WANG Yi, et al. "Bifunctional nanozyme activities of layered double hydroxide derived Co-Al-Ce mixed metal oxides for antibacterial application." Journal of Oceanology and Limnology. Mar. 21, 2020.
- 5. Wang J, Wang Y, et al. "Exploring the bactericidal performance and application of novel mimic enzyme Co4S3." J Colloid Interface Sci. 2020;561:327–337. PMID: 31771873
- 6. YiWangacd, ChaoChenabcd, et al. "Bifunctionalized novel Co-V MMO nanowires: Intrinsic oxidase and peroxidase like catalytic activities for antibacterial application." Applied Catalysis B: Environmental Available online 5 October 2019, 118256. PMID: 31666990
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 424.4 |
| Cas No. | 359010-69-8 |
| Formula | C26H16O6 |
| Synonyms | Hydroxyphenyl fluorescein; 3'-p-(Hydroxyphenyl) fluorescein |
| Solubility | ≤20mg/ml in ethanol; 20mg/ml in DMSO; 20mg/ml in dimethyl formamide |
| Chemical Name | 3'-hydroxy-6'-(4-hydroxyphenoxy)-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one |
| SDF | Download SDF |
| Canonical SMILES | OC(C=C1)=CC=C1OC2=CC3=C(C=C2)C4(C(C=CC=C5)=C5C(O4)=O)C6=CC=C(O)C=C6O3 |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
- View current batch:
Chemical structure
