Hoechst 33342
Catalog No.
A3472
Blue fluorescent dyes for detection of nucleus
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Hoechst 33342 is a blue-fluorescent, cell-permeable DNA probe that readily enters living cells and binds selectively to the A–T rich minor groove of double-stranded DNA, emitting bright fluorescence. Its lipophilic ethyl group enhances plasma-membrane traversal, allowing rapid nuclear labeling without fixation or permeabilization.
So, it is ideal for real-time imaging, flow cytometry, side-population (stem-cell) sorting, apoptosis detection, and high-content drug screens. Compatible with both live and fixed samples, Hoechst 33342 serves as a reliable nuclear counterstain across cell biology, immunology, and neuroscience research.
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| Storage | Store at -20°C |
| M.Wt | 561.93 |
| Cas No. | 875756-97-1 |
| Formula | C27H31Cl3N6O |
| Synonyms | bisBenzimide H 33342;HOE 33342;Hoechst-33342;Hoechst33342 |
| Solubility | insoluble in EtOH; ≥28.7 mg/mL in H2O with gentle warming; ≥46 mg/mL in DMSO |
| Chemical Name | 2'-(4-ethoxyphenyl)-6-(4-methylpiperazin-1-yl)-1H,3'H-2,5'-bibenzo[d]imidazole trihydrochloride |
| SDF | Download SDF |
| Canonical SMILES | CCOC1=CC=C(C2=NC3=C(C=C(C4=NC5=C(C=C(N6CCN(C)CC6)C=C5)N4)C=C3)N2)C=C1.Cl.Cl.Cl |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Chemical structure
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