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Hoechst 33342

Catalog No.
A3472
Blue fluorescent dyes for detection of nucleus
Grouped product items
SizePriceStock Qty
25mg
$55.00
In stock
100mg
$94.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

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Email: [email protected]

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Background

Hoechst 33342 is a blue-fluorescent, cell-permeable DNA probe that readily enters living cells and binds selectively to the A–T rich minor groove of double-stranded DNA, emitting bright fluorescence. Its lipophilic ethyl group enhances plasma-membrane traversal, allowing rapid nuclear labeling without fixation or permeabilization. 
So, it is ideal for real-time imaging, flow cytometry, side-population (stem-cell) sorting, apoptosis detection, and high-content drug screens. Compatible with both live and fixed samples, Hoechst 33342 serves as a reliable nuclear counterstain across cell biology, immunology, and neuroscience research.

Product Citation

Chemical Properties

StorageStore at -20°C
M.Wt561.93
Cas No.875756-97-1
FormulaC27H31Cl3N6O
SynonymsbisBenzimide H 33342;HOE 33342;Hoechst-33342;Hoechst33342
Solubilityinsoluble in EtOH; ≥28.7 mg/mL in H2O with gentle warming; ≥46 mg/mL in DMSO
Chemical Name2'-(4-ethoxyphenyl)-6-(4-methylpiperazin-1-yl)-1H,3'H-2,5'-bibenzo[d]imidazole trihydrochloride
SDFDownload SDF
Canonical SMILESCCOC1=CC=C(C2=NC3=C(C=C(C4=NC5=C(C=C(N6CCN(C)CC6)C=C5)N4)C=C3)N2)C=C1.Cl.Cl.Cl
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

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