In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HNMPA is a tyrosine kinase inhibitor that inhibited both the receptor serine and tyrosine phosphorylation, including insulin receptor tyrosine kinase activity .
Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for growth factors, cytokines, and hormones. The insulin receptor is one of a number of growth factor receptors with intrinsic tyrosine kinase activity that can be activated upon ligands binding .
HNMPA (Hydroxy-2-naphthalenylmethyl Phosphonic Acid) is a tyrosine kinase inhibitor that blocks receptor serine and tyrosine phosphorylation. HNMPA does not affect protein kinase C or cyclic AMP-dependent protein kinase activities. HNMPA inhibited tyrosine kinase activity of autophosphorylated insulin receptor towards poly (Glu4, Tyr) or insulin receptor-(1155-1165) peptide by 82% and 81%, respectively. HNMPA also inhibited autophosphorylation of insulin receptors by 13% + 4.6% in the presence of insulin. HNMPA not only inhibited insulin receptor tyrosine phosphorylation but also effectively decreased insulin receptor serine phosphorylation . In β-cells exposed to high glucose, HNMPA was able to further increase the exe-4-induced insulin secretion .
. Baltensperger K, Lewis RE, Woon CW, et al. Catalysis of serine and tyrosine autophosphorylation by the human insulin receptor. Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):7885-9.
. Moon MJ, Kim HY, Park S, et al. Insulin contributes to fine-tuning of the pancreatic beta-cell response to glucagon-like peptide-1. Mol Cells. 2011 Oct;32(4):389-95.
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Synonyms||Hydroxy-2-naphthalenylmethyl Phosphonic Acid|
|Solubility||≤20mg/ml in ethanol;1mg/ml in DMSO;15mg/ml in dimethyl formamide|
|Chemical Name||P-(hydroxy-2-naphthalenylmethyl)-phosphonic acid|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|