HDAC Inhibitor Drug Screening Kit (Fluorometric)
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Histone deacetylase (HDAC) is an enzyme that removes acetyl group from a histone and plays an important role in regulating gene expression. Inhibition of HDAC can regulate transcription and induce apoptosis or differentiation in cancer cells. However, screening compounds that inhibit HDAC is difficult due to the lack of convenient way for detecting HDAC activity.
The HDAC Inhibitor Drug Screening Kit (Fluorometric) provides a fast and convenient way for screening of compounds for HDAC inhibition by detecting HDAC activity based on fluorescence method that eliminates radioactivity, chromatography or extractions in traditional assays. The assay needs only two easy steps performed on the same microtiter plate. First, the HDAC inhibitor candidates are mixed with HeLa Nuclear Extract and HDAC fluorometric substrate that contains an acetylated lysine side chain. Second, Deacetylation of the HDAC substrate sensitizes the substrate, so that further treatment with the Lysine Developer generates a fluorophore. The fluorophore can be easily detected using a fluorometer or a fluorescence plate reader. The assay is well suitable for high throughput screening applications.
Features & Properties
|Features||Fast fluorescence-based method that eliminates radioactivity, extractions, or chromatography. Simple two-step procedure. Well suited for high throughput screening applications.|
|Storage Conditions||Store at -20℃.|
Quality Control & DataSheet
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|Kit components||HDAC Substrate [Boc-Lys(Ac)-AMC, 4 mM] 10 × HDAC Assay Buffer Lysine Developer HDAC Inhibitor (Trichostatin A, 1 mM) HeLa Nuclear Extract (5 mg/ml)|