In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Furosemide is an inhibitor of Na+/2Cl-/K+ cotransporter .
The Na-K-Cl cotransporter (NKCC) is a protein that acts as an active transporter helping sodium, potassium, and chloride into and out of cells. NKCC1 has important functions in organs that secrete fluids. NKCC2 exists in the kidney and serves to extract sodium, potassium, and chloride from the urine .
Furosemide is an antagonist of Na+/2Cl-/K+ cotransporter. In adult brain tissue, furosemide blocked the epileptiform activity through inhibiting cell swelling, which was concomitant with its inhibition of activity-driven changes of the extracellular space (ECS) . Furosemide inhibited the production and release of cytokines IL-6, IL-8, and TNF-ɑ from peripheral mononuclear cells and exhibited an anti-inflammatory effect .
In patients with liver cirrhosis, furosemide inhibited the natriuretic effect and increased the levels of atrial natriuretic factor (ANF) and circulating endothelin. In neonates, furosemide significantly increased the urinary excretion of aldosterone and endothelin-1 .
. Hochman DW. The extracellular space and epileptic activity in the adult brain: explaining the antiepileptic effects of furosemide and bumetanide. Epilepsia, 2012, 53 Suppl 1: 18-25.
. Chen H, Sun D. The role of Na-K-Cl co-transporter in cerebral ischemia. Neurol Res, 2005, 27(3): 280-286.
. Prandota J. Furosemide: progress in understanding its diuretic, anti-inflammatory, and bronchodilating mechanism of action, and use in the treatment of respiratory tract diseases. Am J Ther, 2002, 9(4): 317-328.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||≥14.9mg/mL in DMSO|
|Chemical Name||4-chloro-2-(furan-2-ylmethylamino)-5-sulfamoylbenzoic acid|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|